ZFIN ID: ZDB-FIG-190708-50
Kujawski et al., 2019 - penner/lgl2 is required for the integrity of the photoreceptor layer in the zebrafish retina. Biology Open   8(4) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5

Fig. 3

pen/lgl2 mutant distal retina displays no significant abnormalities in polarity or cellular morphology.Immunostaining of transverse retinal sections at 5 dpf. (A,C,E,G,I,K,M,O) Sibling and (B,D,F,H,J,L,N,P) mutant fish. (A–B′) Zpr1, (C–D′) WGA, (E–F′) Zpr2, (G–H′) Crb2a, (I–J′) aPKC, (K–L′) Moe, (M–N′) ZO-1 and (O–P′) N-cadherin immunostaining of distal retina. PRC, photoreceptor layer; INL, inner nuclear layer; OS, outer segment; OPL, outer plexiform layer; RPE, retinal pigment layer; OLM, outer limiting membrane. Scale bar: 10 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crb2a zs-4 AB standard conditions Day 5 eye photoreceptor cell subapical part of cell IHC
zs-4 Day 5 photoreceptor inner segment layer subapical part of cell IHC
zs-4 llgl2to6/to6 standard conditions Day 5 eye photoreceptor cell subapical part of cell IHC
zs-4 Day 5 photoreceptor inner segment layer subapical part of cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-prkcz IHC AB standard conditions Day 5 eye photoreceptor cell subapical part of cell
IHC Day 5 photoreceptor inner segment layer subapical part of cell
IHC llgl2to6/to6 standard conditions Day 5 eye photoreceptor cell subapical part of cell
IHC Day 5 photoreceptor inner segment layer subapical part of cell
Ab1-tjp1 IHC AB standard conditions Day 5 outer limiting membrane
IHC llgl2to6/to6 standard conditions Day 5 outer limiting membrane
zpr-1 IHC AB standard conditions Day 5 retinal photoreceptor layer
IHC llgl2to6/to6 standard conditions Day 5 retinal photoreceptor layer
zpr-2 IHC AB standard conditions Day 5 retinal pigmented epithelium
IHC llgl2to6/to6 standard conditions Day 5 retinal pigmented epithelium
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open