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ZFIN ID: ZDB-FIG-181114-4
Sun et al., 2018 - Maternal Ybx1 safeguards zebrafish oocyte maturation and maternal-to-zygotic transition by repressing global translation. Development (Cambridge, England)   145(19) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: 128-cell
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-cell to Adult

Fig. 2

Characterization of Mybx1 mutant phenotype. (A) WT and Mybx1 embryos with chorions at 0.5 hpf. (B) Measurement of chorion diameters and chorion elevation distances at 0.5 hpf. ***P<0.001; n=33; Welch's t-test. (C) Representative images showing labelling of cortical granules (CG) in WT and ybx1 mutant eggs fixed at 20 min post-activation (mpa). Lateral views of eggs are shown. Eggs observed: WT n=23, mutant n=28. (D) Early stage-III oocytes, early stage-IV oocytes and ovulated stage-V eggs from WT and Zybx1 female fish. Arrowheads indicate germinal vesicles (oocyte nuclei) in the centre of stage-III oocytes and closer to the cortex in stage-IV oocytes. (E) WT and ybx1 mutant oocytes before and after in vitro maturation. Insets show enlarged regions of the yolk. Relative opaqueness is seen in ybx1 mutants. (F) SDS-PAGE and Coomassie staining of major yolk proteins of stage-III/IV oocytes and stage-V eggs. The higher and lower molecular weight yolk proteins (HYP and LYP) are indicated by the arrowhead and arrow, respectively. HYP/LYP ratios were calculated to represent yolk protein cleavage levels. (G) Major yolk protein cleavage in oocytes before and after in vitro maturation. Three independent experiments were performed for F and G. (H,I) Whole-mount DAPI staining of WT and Mybx1 embryos at 1.5 hpf (H) and 2.25 hpf (I) shown in animal pole views. (J) DAPI staining and NPC immunofluorescence in 2.25 hpf embryos. (K,L) β-Catenin membrane immunofluorescence in 1.5 hpf (K) and 2.25 hpf (L) embryos shown in animal pole views. (M) α-Tubulin immunofluorescence in 1.5 hpf and 2.25 hpf embryos shown in lateral views. Numbers of observations are indicated in H-M. H shows inverted fluorescence microscopic images. I,K,M show maximum intensity projections of confocal z-slices. J and L show individual confocal planes. Scale bars: 200 μm in A,C-E,H,I,K-M; 5 μm in J.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-nucleoporin IHC TU standard conditions 128-cell blastomere nuclear pore
IHC ybx1tsu3d5i/+(TU) standard conditions 128-cell Not Detected blastomere nuclear pore
Ab2-ctnnb1 IHC TU standard conditions 16-cell blastomere plasma membrane
IHC 128-cell blastomere plasma membrane
IHC ybx1tsu3d5i/+(TU) standard conditions 128-cell blastomere plasma membrane
IHC 16-cell blastomere plasma membrane
Ab3-tuba IHC TU standard conditions 16-cell blastomere tubulin complex
IHC 128-cell blastomere tubulin complex
IHC ybx1tsu3d5i/+(TU) standard conditions 16-cell blastomere tubulin complex
IHC 128-cell blastomere tubulin complex
Phenotype Details
Fish Conditions Stage Phenotype
ybx1tsu3d5i/+(TU) standard conditions 1-cell chorion decreased diameter, abnormal
1-cell chorion decreased elevation, abnormal
1-cell cortical granule exocytosis decreased occurrence, abnormal
1-cell fertilized egg cortical granule increased amount, abnormal
128-cell blastomere nuclear pore ab1-nucleoporin labeling absent, abnormal
128-cell blastomere nuclear pore absent, abnormal
16-cell blastomere nucleus broken, abnormal
128-cell blastomere nucleus broken, abnormal
16-cell blastomere plasma membrane structure, abnormal
128-cell blastomere plasma membrane structure, abnormal
16-cell cleavage furrow formation decreased occurrence, abnormal
128-cell cleavage furrow formation decreased occurrence, abnormal
16-cell tubulin complex assembly decreased occurrence, abnormal
128-cell tubulin complex assembly decreased occurrence, abnormal
ybx1tsu3d5i/tsu3d5i(TU) standard conditions Adult oocyte maturation decreased occurrence, abnormal
Adult oocyte stage V immature, abnormal
Adult oocyte stage V opaque, abnormal
Adult oocyte stage V proteolysis decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development