PUBLICATION

Maternal Ybx1 safeguards zebrafish oocyte maturation and maternal-to-zygotic transition by repressing global translation

Authors
Sun, J., Yan, L., Shen, W., Meng, A.
ID
ZDB-PUB-180824-2
Date
2018
Source
Development (Cambridge, England)   145(19): (Journal)
Registered Authors
Meng, Anming
Keywords
Embryogenesis, Maternal-to-zygotic transition, Oocyte maturation, Translational repression, Ybx1, Zebrafish
Datasets
GEO:GSE108924
MeSH Terms
  • Animals
  • Base Sequence
  • Cell Differentiation*
  • Cell Proliferation
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Female
  • Fertilization
  • Gene Expression Regulation, Developmental
  • Genetic Pleiotropy
  • Models, Biological
  • Mutation/genetics
  • Oocytes/cytology*
  • Oocytes/metabolism*
  • Phenotype
  • Protein Biosynthesis*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Unfolded Protein Response
  • Y-Box-Binding Protein 1/genetics
  • Y-Box-Binding Protein 1/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Zygote/metabolism*
PubMed
30135188 Full text @ Development
Abstract
Maternal mRNAs and proteins dictate early embryonic development before zygotic genome activation. In the absence of transcription, elaborate control of maternal mRNA translation is of particular importance for oocyte maturation and early embryogenesis. By analyzing zebrafish ybx1 mutants with a null allele, we demonstrate an essential role of maternal ybx1 in repressing global translation in oocytes and embryos. Loss of maternal Ybx1 leads to impaired oocyte maturation and egg activation. Maternal ybx1 (Mybx1) mutant embryos fail to undergo normal cleavage and the maternal-to-zygotic transition (MZT). Morpholino knockdown of ybx1 also results in MZT loss and epiboly failure, suggesting the postfertilization requirement of Ybx1. In addition, elevated global translation level and the unfolded protein response were found in Ybx1-depleted embryos. Supplementing translational repression by eIF4E inhibition markedly rescues the Mybx1 phenotype. Mechanistically, Ybx1 in embryos may associate with processing body components and repress translation when tethered to target mRNAs. Collectively, our results identify maternal Ybx1 as a global translational repressor required for oocyte maturation and early embryogenesis.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes