FIGURE

Fig. 7

ID
ZDB-FIG-181114-7
Publication
Sun et al., 2018 - Maternal Ybx1 safeguards zebrafish oocyte maturation and maternal-to-zygotic transition by repressing global translation
Other Figures
All Figure Page
Back to All Figure Page
Fig. 7

Translational inhibition ameliorates the Mybx1 phenotype. (A) Western blot of Ybx1, GFP-Eif4ebp1-TTAA and Gapdh protein expression in WT, Mybx1 and Mybx1+TTAA embryos. (B) Bright-field images showing representative Mybx1 and Mybx1+TTAA embryos with chorions after fertilization. (C) Chorion elevations of individual 0.5 hpf fertilized eggs from a single Zybx1 or Zybx1+TTAA female. Each dot or circle represents one egg's value. ***P<0.001; Welch's t-test. (D) Average chorion elevations of fertilized eggs from different females. Six females of each group, 50-100 eggs per female, were analyzed. Each circle represents the average of all observed eggs from a single female. *P<0.05; Welch's t-test. (E) Coomassie staining of major yolk proteins of ybx1 mutant and ybx1+TTAA stage-V eggs. HYP/LYP ratios were calculated to display yolk protein cleavage levels. (F) Major yolk protein cleavage in oocytes before and after in vitro maturation. (G) Bright-field images of Mybx1 and Mybx1+TTAA gastrulas. (H) The percentage of epiboly restored Mybx1+TTAA embryos from different females. Epiboly initiation is extremely rare in Mybx1 mutants. *P<0.05; n=6; Welch's t-test. (I,J) qRT-PCR detection of partially restored maternal mRNA clearance (I) and zygotic gene transcription (J) in Mybx1+TTAA embryos, compared with Mybx1 mutants. WT results are also presented. *P<0.05, **P<0.01; n=3; Student's t-test. Scale bars: 200 μm.

Expression Data
Genes:
Fish:
Anatomical Term:
Stage: Shield

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: 1-cell to Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development