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ZIRC
ZFIN ID: ZDB-FIG-181004-15
Bickers et al., 2018 - Zebrafish snai2 mutants fail to phenocopy morphant phenotypes. PLoS One   13:e0202747 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Long-pec

Fig. 1

Snai2 endogenous expression in wild-type embryos.

Expression of snai2 was analyzed via whole mount in situ hybridization at 14 hpf (A), 18 hpf (B), and 24 hpf (C). Embryos were cryosectioned post-in situ at 18 hpf (D) and 24 hpf (F). Simplified schematics are provided (E and G). Double fluorescent in situ for snai2 with endothelial markers fli1a at 14 hpf (H) and etsrp at 26 hpf (I) was performed. Insets show a close-up view of the PLM. QPCR was used to compare snai2 enrichment within double positive HSPCs sorted from Tg(CD41:GFP/kdrl:mCherry) on 2 dpf to the rest of the embryo. Markers cmyb and kdrl, which should be enriched in this population, are displayed alongside for comparison. N: notochord; NT: neural tube; S: somite.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
etsrp AB standard conditions Prim-5 endothelial cell ISH
fli1 AB standard conditions 10-13 somites endothelial cell ISH
snai2 AB standard conditions 10-13 somites Not Detected endothelial cell ISH
10-13 somites mesoderm ISH
10-13 somites nervous system ISH
10-13 somites somite ISH
14-19 somites somite ventral region ISH
Prim-5 Not Detected endothelial cell ISH
Prim-5 somite ventral region ISH
la2Tg; s896Tg standard conditions Long-pec Not Detected endothelial cell RTPCR
Long-pec Not Detected hematopoietic multipotent progenitor cell RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One