FIGURE

Fig. S5

ID

ZDB-FIG-180914-11

Publication

Matejčić et al., 2018 - A non-cell-autonomous actin redistribution enables isotropic retinal growth

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

Hdac1−/− characterization.

(A) Phalloidin (green) and PH3 antibody (magenta) staining of F-actin and mitotic cells in about 70 hpf hdac1−/− retinal PSE. Apical mitoses, junctional belts, and basal actin accumulation are preserved in the folded tissue. (B) Laminin-alpha1 and Collagen IV (B’) antibody staining of wild-type and hdac1−/− retinal tissues around 48 hpf. An intact basal lamina underlies the basal surface of the hdac1−/− retinal PSE. (C) Tissue thickness does not increase in Hdac1 morpholino-injected or TSA-treated retinal PSE (42 hpf). (D) Phalloidin staining of approximately 72 hpf hdac1−/−. Epithelial folds form throughout the retinal PSE. (E) Tg(Ath5::GFP) retinas (red) treated with TSA at 24 hpf for 24 h and stained with phalloidin (green) and DRAQ5 (blue). The medium was replaced after 24 h. The PSE differentiates by 70 hpf despite tissue shape perturbed by folds. Consequently, neuronal layers are perturbed, as well. Scale bars: 50 μm for all images. DRAQ5, deep red anthraquinone; Hdac1, histone-deacetylase 1; hpf, hours post fertilization; PH3, phosphorylated histone H3; PSE, pseudostratified epithelium; TSA, Trichostatin-A.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.