ZFIN ID: ZDB-FIG-180911-9
Uribe et al., 2018 - In vivo analysis of cardiomyocyte proliferation during trabeculation.. Development (Cambridge, England)   145(14) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Days 7-13

Fig. 5

The TGFβ pathway ligand Inhbaa promotes trabecular cardiomyocyte proliferation. (A-H) Quantification and images of cardiomyocyte proliferation in the Tg(myl7:inhbaa-2A-H2B-EGFP) line after EdU incubation from 72 to 96 hpf (A-D) and from 96 to 120 hpf (E-H) in the two myocardial layers; myosin heavy chain staining labels cardiomyocytes. Overexpression of inhbaa drives cardiomyocyte proliferation in the trabecular, but not the compact, layer; each point represents a heart (D,H). (I-J″) Evaluation of the trabecular phenotype in the Tg(myl7:inhbaa-2A-H2B-EGFP) line at 10 dpf; Tg(myl7:MKATE-CAAX) expression labels cardiomyocyte membranes. A denser trabecular network is observed in inhbaa-overexpressing cardiomyocytes (compare cross-section in I′ with J′, and 3D surface rendering I″ with J″). Six control and seven inhbaa-overexpressing larvae are imaged. Data are mean±s.d., *P=0.041 in C, **P=0.0025 in D, **P=0.0011 in G, ***P=0.0007 in H; Student's two-tailed t-test; each experiment was repeated twice. Scale bars: 20 µm. Scale bars in A,E,I,J apply to B,F,I′,J′, respectively.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
MKATE2 sd11Tg control Days 7-13 cardiac muscle cell membrane IFL
sd11Tg ; bns146Tg control Days 7-13 cardiac muscle cell membrane IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT control Day 4 cardiac muscle cell
IHC Day 5 cardiac muscle cell
IHC bns146Tg control Day 4 cardiac muscle cell
IHC Day 5 cardiac muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
bns146Tg control Day 4 cardiac muscle cell proliferation increased occurrence, abnormal
Day 4 trabecular layer cardiac muscle cell proliferation increased occurrence, abnormal
Day 5 cardiac muscle cell proliferation increased occurrence, abnormal
Day 5 trabecular layer cardiac muscle cell proliferation increased occurrence, abnormal
sd11Tg ; bns146Tg control Days 7-13 heart trabecula formation increased rate, abnormal
Days 7-13 trabecular layer cardiac muscle cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development