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ZFIN ID: ZDB-FIG-180827-14
Ferrero et al., 2018 - Embryonic Microglia Derive from Primitive Macrophages and Are Replaced by cmyb-Dependent Definitive Microglia in Zebrafish. Cell Reports   24:130-141 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 2

pMFs Are the Unique Source of MG in the Vertebrate Embryo

(A–C) Expression of the MG marker apoeb (red quadrants) in the brains of cmybnull (A), tfecnull (B), alk8null(lost-a-fin) (C), and their sibling control animals at the indicated developmental stages. For (A), cmybnull embryos are identified by the concomitant absence of lymphoid-specific rag1 (arrowheads) transcripts in the thymus. Scale bar: 50 μm.

(A′–C′) Quantification of apoeb+ MG in embryos of indicated genotypes. Each symbol represents a single embryo. Error bars represent mean ± SEM.

(D) Strategy for transient fate mapping of pMFs in vivo.

(E) Flow cytometry showing absence of kaede-Red expression in non-photoconverted embryos (left panel), whereas cells expressing both forms of kaede were present and sortable in the photoconverted embryos (right panel).

(F) qPCR analyses for apoeb and csf1ra on mpeg1:kaede-Green cells sorted from control and photoconverted embryos, and on mpeg1:kaede-Green/Red cells from photoconverted embryos. The color code matches the gates on the FACS plots. Error bars represent mean ± SD (n = 3 experiments).

(G) Sagittal sections of E10.5 Runx1null embryos and their littermates (upper body) immunostained for CD31 (vascular) and CD45 (hematopoietic) markers, with DAPI used as a nuclear counterstain. Tile-scanned images show that Runx1null mutants are completely devoid of CD45+ MG in the neuroepithelium and cephalic mesenchyme (n = 3). Scale bar: 500 μm.

See also Figures S1–S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
apoeb WT standard conditions Protruding-mouth brain microglial cell ISH
Day 4 brain microglial cell ISH
Day 6 brain microglial cell ISH
acvr1ltm110b/tm110b standard conditions Protruding-mouth Not Detected brain microglial cell ISH
mybt25127/t25127 standard conditions Day 6 brain microglial cell ISH
tfecug103/ug103 standard conditions Day 4 brain microglial cell ISH
rag1 WT standard conditions Day 6 thymus ISH
mybt25127/t25127 standard conditions Day 6 Not Detected thymus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
acvr1ltm110b/tm110b standard conditions Protruding-mouth brain microglial cell absent, abnormal
Protruding-mouth brain microglial cell apoeb expression absent, abnormal
mybt25127/t25127 standard conditions Day 6 thymus rag1 expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.