header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180627-36
Sedykh et al., 2017 - Zebrafish Rfx4 controls dorsal and ventral midline formation in the neural tube. Developmental dynamics : an official publication of the American Association of Anatomists   247(4):650-659 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 3

Dorsal hindbrain is aberrant in rfx4 mutant embryos by 24 hpf. WISH was used to visualize gene expression patterns in rfx4uw8013 homozygotes vs rfx4uw8013/ + sibling embryos, which develop normally, at 24 hpf. zic2a expression is reduced in the mutant dorsal hindbrain by 18 s (A,B) and remains depleted at 24 hpf (C,D). E,F: wnt3a expression is unaffected in midbrain, but reduced in hindbrain of mutant embryos. G,H: pax7a expression is unaffected in the alar plate of the midbrain and hindbrain in mutant embryos. I,J: dbx1a expression in the diencephalon and in the midbrain/hindbrain basal plate is likewise unaffected in mutants. mb, midbrain; hb, hindbrain; open arrowheads, optic stalk expression; arrows, depleted hindbrain expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbx1a rfx4uw8013/+ standard conditions Prim-5 basal plate midbrain region ISH
Prim-5 diencephalon ISH
rfx4uw8013/uw8013 standard conditions Prim-5 basal plate midbrain region ISH
Prim-5 diencephalon ISH
pax7a rfx4uw8013/+ standard conditions Prim-5 hindbrain dorsal region ISH
Prim-5 midbrain ISH
rfx4uw8013/uw8013 standard conditions Prim-5 hindbrain dorsal region ISH
Prim-5 midbrain ISH
wnt3a rfx4uw8013/+ standard conditions Prim-5 hindbrain dorsal region ISH
Prim-5 midbrain ISH
rfx4uw8013/uw8013 standard conditions Prim-5 hindbrain dorsal region ISH
Prim-5 midbrain ISH
zic2a rfx4uw8013/+ standard conditions Prim-5 hindbrain dorsal region ISH
Prim-5 spinal cord ISH
rfx4uw8013/uw8013 standard conditions Prim-5 hindbrain dorsal region ISH
Prim-5 spinal cord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rfx4uw8013/uw8013 standard conditions Prim-5 hindbrain dorsal region wnt3a expression decreased amount, abnormal
Prim-5 hindbrain dorsal region zic2a expression decreased amount, abnormal
Prim-5 spinal cord zic2a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.