ZFIN ID: ZDB-FIG-180412-5
Razaghi et al., 2017 - hace1 influences zebrafish cardiac development via ROS-dependent mechanisms. Developmental dynamics : an official publication of the American Association of Anatomists   247(2):289-303 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4 ROS levels are elevated throughout the embryo and in particular in the heart following hace1 knockdown. ROS is labeled by green fluorescence generated with oxidation of 2',7'‐ DCFH‐DA. A: AB embryos injected with control morpholino demonstrate baseline levels of ROS in the whole organism. B: AB embryo following hace1 knockdown exhibits elevated levels of ROS throughout the embryo, in particular in cardiac structures (indicated by the white arrow). Scale bar = 250 µm for panels A and B. C: Confocal imaging of Tg(cmlc2::mCherry‐Ras)sd21 embryo injected with control morpholino demonstrates a normal two‐chamber heart in red with baseline levels of ROS. D: Confocal imaging of Tg(cmlc2::mCherry‐Ras)sd21 embryo injected with hace1 morpholino demonstrates a cardiac‐looping phenotype and accumulation of ROS in the heart. Scale bar = 100 µm for panels C and D. All embryos are 48 hpf and visualized laterally.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-hace1 standard conditions Long-pec whole organism reactive oxygen species increased amount, abnormal
sd21Tg standard conditions Long-pec heart looping process quality, normal
Long-pec heart reactive oxygen species amount, normal
sd21Tg + MO1-hace1 standard conditions Long-pec heart looping disrupted, abnormal
Long-pec heart reactive oxygen species increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.