ZFIN ID: ZDB-FIG-180412-8
Razaghi et al., 2017 - hace1 influences zebrafish cardiac development via ROS-dependent mechanisms. Developmental dynamics : an official publication of the American Association of Anatomists   247(2):289-303 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 6 nox1 and nox2 knockdown or treatment with ROS and NADPH oxidase inhibitors rescues the cardiac phenotype by decreasing ROS levels. Lateral views of Tg(cmlc2::mCherry‐Ras)sd21 embryos stained with DCFH‐DA (5 μM) at 48 hpf following morpholino knockdown of hace1 (A), injection of control morpholino (B), morpholino knockdown of nox1 (C), nox2 (D), nox1/nox2 (E), and knockdown combination of hace1/nox1/nox2 (F). Co‐injection of hace1 MO + nox1/nox2 MOs rescues the abnormal cardiac phenotypes demonstrating the lowest levels of ROS and normal cardiac structure (F). Quantification of abnormal cardiac phenotypes in hace1 morphants following treatment with (G) NAC, ** P < 0.0001, and (H) Apo, ** P = 0.0098. The number on top of each bar represents the total number of embryos in the group. Scale bar = 250 µm. DCFH‐DA = 2',7'‐dichlorofluorescein diacetate.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sd21Tg + MO1-hace1 standard conditions Long-pec heart looping disrupted, abnormal
Long-pec heart reactive oxygen species increased amount, abnormal
sd21Tg + MO1-hace1 + MO1-nox1 + MO2-cybb standard conditions Long-pec heart looping process quality, ameliorated
Long-pec whole organism reactive oxygen species amount, ameliorated
sd21Tg + MO1-nox1 standard conditions Long-pec heart looping process quality, normal
Long-pec whole organism reactive oxygen species decreased amount, abnormal
sd21Tg + MO1-nox1 + MO2-cybb standard conditions Long-pec heart looping process quality, normal
Long-pec whole organism reactive oxygen species decreased amount, abnormal
sd21Tg + MO2-cybb standard conditions Long-pec heart looping process quality, normal
Long-pec whole organism reactive oxygen species decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.