Fig. 3

Zhang et al., 2017 - Ribosomal Proteins Rpl22 and Rpl22l1 Control Morphogenesis by Regulating Pre-mRNA Splicing
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Fig. 3

The C&E Defects in Like1 Morphants Can Be Rescued by Re-establishing smad2 Signaling (A) Schematic of the morpholino used to induce exon 9 skipping (S2-i8e9-MO). (B) Immunoblotting of detergent extracts reveals a reduction in total and phospho-Smad2 protein expression in the S2-i8e9-morphants. (C–E) S2-i8e9-MO induction of smad2 mis-splicing phenocopied the C&E defects caused by Like1 knockdown, as indicated by altered morphology (C, red arrow) and alterations in ntl/hgg1/dlx3b and lefty1 expression and distribution, as measured by in situ hybridization (D, 10 hpf, red arrows, anterior dorsal view; E, 16 hpf, lateral view). (F–H) mRNA encoding constitutively activated smad2 (Ca-Smad2, 20 pg) was used for injection alone or co-injected with Like1-A-MO. Embryo morphology (F) as well as the abnormal distribution of ntl (G, red arrows) and myod1 (H, red arrows) at 10 hpf can be rescued by ectopic expression of Ca-Smad2. All embryos are dorsal view with the anterior on top at 10 hpf. All results are representative of at least three experiments performed. See also Figure S3.

Expression Data
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to 14-19 somites

Expression Detail
Antibody Labeling
Phenotype Data
Knockdown Reagents:
Observed In:
Stage Range: Bud to 14-19 somites

Phenotype Detail
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