FIGURE

Fig. 4

ID

ZDB-FIG-180403-4

Publication

Zhang et al., 2017 - Ribosomal Proteins Rpl22 and Rpl22l1 Control Morphogenesis by Regulating Pre-mRNA Splicing

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Fig. 4

The Regulation of smad2 Pre-mRNA Splicing during Gastrulation by Rpl22 and Like1 Is Associated with Their Retention in the Nucleus (A and B) Subcellular location of epitope-tagged Rpl22 and Like1 at 10 hpf (A) and 24 hpf (B). mRNA (100 pg) encoding HA-zRpl22 (HA/L22) and HA-zLike1 (HA/L1) was co-injected with mRNA encoding mCherry-CAAX into one-cell stage embryos and visualized by HA antibody immunostaining. mCherry-CAAX marked the cell membrane, and DAPI marked the nucleus. The red scale bar represents 10 μm. (C) RT-PCR detection of smad2 mis-splicing. Following Like1 MO injection, smad2 mis-splicing was assessed by RT-PCR in 10 and 24 hpf Like1 morphants. All results are representative of at least three experiments performed. See also Figure S4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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