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ZFIN ID: ZDB-FIG-180109-20
Niklaus et al., 2017 - Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate Transporters. eNeuro   4(3) Full text @ eNeuro
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 3

Confirmation of knockdown. A–C, Immunostaining of EAAT2a on WT (A) and EAAT2a morphant [B, 1.3 ng EAAT2a morpholino (MO) 1; C, 1.8 ng EAAT2a MO 2] retinal sections (5 dpf). D, Box-and-whisker plot of analysis of fluorescence of WT, EAAT2a MO 1, and EAAT2a MO 2 injected animals stained with anti-EAAT2a antibody. Statistical analysis reveals a highly significant (p < 0.001) reduction of fluorescence for both MOs. E–G, Retinal sections of WT (E) and EAAT2b morphant (F, 1.8 ng EAAT2b MO 1; G, 9 ng EAAT2b MO 2) larvae stained with anti-EAAT2b antibody. H, Fluorescence was measured in the OPL, and background fluorescence (taken from area in INL) was subtracted. Fluorescence of WT and morphant immunostaining is plotted in a box-and-whisker plot and shows a significant (p < 0.01) and slightly significant (p < 0.05) decrease in fluorescence in animals injected with 1.8 ng EAAT2b MO 1 and 9 ng EAAT2b MO 2, respectively. EAAT2a WT, n = 6; EAAT2a MO 1, n = 8; EAAT2a MO 2, n = 8; EAAT2b WT, n = 10; EAAT2b MO 1, n = 10; EAAT2b MO 2, n = 10. Scale bar in A is 30 μm; also applies to B and C. Scale bar in E is 10 μm; also applies to F and G.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
slc1a2a Ab1-slc1a2a WT standard conditions Day 5 retinal outer plexiform layer IHC
Ab1-slc1a2a WT + MO1-slc1a2a standard conditions Day 5 retinal outer plexiform layer IHC
Ab1-slc1a2a WT + MO2-slc1a2a standard conditions Day 5 retinal outer plexiform layer IHC
slc1a2b Ab1-slc1a2b WT standard conditions Day 5 retina IHC
Ab1-slc1a2b WT + MO2-slc1a2b standard conditions Day 5 retina IHC
Ab1-slc1a2b WT + MO3-slc1a2b standard conditions Day 5 retina IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-slc1a2a standard conditions Day 5 retinal outer plexiform layer slc1a2a expression decreased amount, abnormal
WT + MO2-slc1a2a standard conditions Day 5 retinal outer plexiform layer slc1a2a expression decreased amount, abnormal
WT + MO2-slc1a2b standard conditions Day 5 retina slc1a2b expression decreased amount, abnormal
WT + MO3-slc1a2b standard conditions Day 5 retina slc1a2b expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eNeuro for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ eNeuro