ZFIN ID: ZDB-FIG-180109-19
Niklaus et al., 2017 - Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate Transporters. eNeuro   4(3) Full text @ eNeuro
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult

Fig. 2

Protein expression of EAAT2 paralogs. A–F, Double immunostaining of EAAT2a (green) and glutamine synthetase (magenta) in adult (A) and larval (5 dpf; D) retinal sections confirm expression of EAAT2a in Müller glia cells. Separated channels are shown in B (adult), E (5 dpf; EAAT2a, green channel only), C (adult), and F (5 dpf; glutamine synthetase, magenta channel only). Scale bar in A is 30 µm; also applies to B and C. Scale bar in D is 50 µm; also applies to E and F. G–N, EAAT2b protein is expressed in a dotted manner in the outer plexiform layer (OPL) in all cone pedicles, but it is not expressed in rods. EAAT2b antibody staining (magenta) on adult retinal sections stained with Zpr-1 (red-green double cones, G) and on retinal sections of zebrafish expressing GFP in blue cones (H), UV cones (I), and rods (J) confirms that EAAT2b is cone specific and is spared from rod spherules. K–M show zoom-ins of the cone pedicles expressing EAAT2b (magenta) in red-green double cones (K), blue cones (L), and UV cones (M). N shows larval (5 dpf) expression of EAAT2b in magenta together with a nuclear counterstain (DAPI, blue). Scale bars in G–J are 7 µm. Scale bars in K–M are 2 µm. Scale bar in N is 30 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP kj11Tg standard conditions Adult blue sensitive photoreceptor cell IFL
Adult retinal cone cell IFL
kj2Tg standard conditions Adult retinal rod cell IFL
kj9Tg standard conditions Adult retinal cone cell IFL
Adult UV sensitive photoreceptor cell IFL
slc1a2a Ab1-slc1a2a WT standard conditions Day 5 retinal outer plexiform layer IHC
Ab1-slc1a2a Adult green sensitive photoreceptor cell IHC
Ab1-slc1a2a Adult red sensitive photoreceptor cell IHC
Ab1-slc1a2a Adult retinal cone cell cone cell pedicle IHC
Ab1-slc1a2a Adult retinal outer plexiform layer IHC
Ab1-slc1a2a Adult Not Detected retinal rod cell IHC
Ab1-slc1a2a kj11Tg standard conditions Adult blue sensitive photoreceptor cell IHC
Ab1-slc1a2a Adult retinal cone cell presynapse IHC
Ab1-slc1a2a kj2Tg standard conditions Adult retinal cone cell presynapse IHC
Ab1-slc1a2a Adult Not Detected retinal rod cell IHC
Ab1-slc1a2a kj9Tg standard conditions Adult retinal cone cell presynapse IHC
Ab1-slc1a2a Adult UV sensitive photoreceptor cell IHC
slc1a2b Ab1-slc1a2b WT standard conditions Day 5 Muller cell plasma membrane IHC
Ab1-slc1a2b Adult Muller cell plasma membrane IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-glul IHC WT standard conditions Day 5 blue sensitive photoreceptor cell
IHC Day 5 retinal cone cell
IHC Adult blue sensitive photoreceptor cell
IHC Adult retinal cone cell
zpr-1 IHC WT standard conditions Adult green sensitive photoreceptor cell
IHC Adult red sensitive photoreceptor cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal eNeuro for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ eNeuro