Fig. 2

Juárez-Morales et al., 2016 - Evx1 and Evx2 specify excitatory neurotransmitter fates and suppress inhibitory fates through a Pax2-independent mechanism
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Expression of evx and eng1b genes in evx1;evx2 double mutant embryos. Lateral views of zebrafish spinal cord at 24 h (a-f, i & j) or 30 h (g, h, k & l ). Anterior left, dorsal up. a-j in situ hybridization for each gene indicated. e-h strong ventral expression is in muscle pioneer cells, expression in more individual dorsal cells corresponds to spinal cord V1 cells. k & l immunohistochemistry for Evx2. m-p Average number of cells (y-axis) expressing indicated marker in spinal cord region adjacent to somites 6-10 in WT embryos and evx1 and evx2 single and double mutants (x-axis) at 24 h (m-o) or 30 h (p). Values are shown as mean +/- standard deviation (values are provided in Table 1). There are no evx2-positive cells in the double mutants (n). In each case at least 5 embryos were counted, except for eng1b where 4 embryos were counted. Statistically significant differences (P < 0.05) from WT values are indicated with brackets and stars. P values for these and other comparisons (e.g differences between single and double mutants) are provided in Table 1. Scale bar: 50 μm (a-l)

Expression Data
Anatomical Terms:
Stage Range: Prim-5 to Prim-15

Expression Detail
Antibody Labeling
Phenotype Data
Observed In:
Stage: Prim-5

Phenotype Detail
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.