Fig. 2

Dvr1 acts downstream of Setdb2 to affect C&E movements. (A) The relative expression levels of dvr1 in control and setdb2 morphant embryos from 2.5 hpf to 10 hpf. The upregulation of dvr1 was firstly detected at 5.5 hpf in the setdb2 morphants. (B) The relative expression levels of setdb2 and dvr1 from 2.5 hpf to 10 hpf in wild-type embryos. (C) The expression of the setdb2 gene was unaffected in two types of dvr1 morphants compared to the control. Gapdh expression was analyzed as a control in quantitative real-time PCR. (D-L) dvr1 mo^ATG partially rescued the C&E movement defects of setdb2 morphants. The embryos were injected at the one-cell stage with 16 ng setdb2 control mo, 16 ng setdb2 mo or 16 ng setdb2 mo plus 4 ng dvr1 mo^ATG. Whole-mount in situ hybridization of the expression patterns of fgf8a and ntla were performed at the indicated developmental stages. L1, the width of the presumptive hindbrain at 10 hpf; L2 and L3, the width and length of the notochord. Dorsal views of embryos in G-I were flat-mounted. Embryos were dorsal views in left side of D–F with head to the top and lateral views in right side of D-F. Embryos were lateral views in upper of J–L and dorsal views in nether of J–L with head to the left. (M) Statistical analyses of the expression patterns of fgf8a and ntla were performed. Asterisks indicate statistically significant differences (**, P<0.01; <****, P<0.0001).