ZFIN ID: ZDB-FIG-170908-29
Brunt et al., 2017 - Wnt signalling controls the response to mechanical loading during Zebrafish joint development. Development (Cambridge, England)   144(15):2798-2809 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 4

Immobilisation and Wnt16 knockdown affects cell proliferation and migration at the medial region of the jaw joint between 3 and 5 dpf. (A-D′) Tg(Sox10:GAL4-VP16) and Tg(UAS:Kaede) transgenic line drives expression of Kaede protein (green) in the cartilage of control (A), anaesthetised (B), control-injected (C) and Wnt16 morpholino (MO)-injected (D) zebrafish. Maximum projections of the jaw joint from stacks of tiff images (A,B,C,D) and single slice/substacks through the same jaw joint to show cell morphology (A′,B′,C′,D′) are represented. At the jaw joint, medially located Kaede-expressing cells are photoconverted to red Kaede at 3 dpf (left panels). Right panels show jaw joints from the same larva re-imaged at 5 dpf. Photoconverted cells appear red/orange owing to presence of photoconverted red Kaede and expression of newly made green Kaede protein under control of the sox10 promoter. (E) Percentage increase in total area of cells expressing photoconverted red Kaede between 3 and 5 dpf in control, anaesthetised, control-injected and Wnt16 MO-injected zebrafish jaw joints (n=17, 18, 10 and 8 joints, respectively). (F) Percentage increase in number of cells expressing photoconverted red Kaede between 3 and 5 dpf in control, anaesthetised, control-injected and Wnt16 MO-injected zebrafish jaw joints (n=17, 16, 10 and 10 joints, respectively). Kruskal–Wallis tests were performed for E,F. ns, not significant, *P≤0.05, ***P≤0.001. Data are mean and 95%CI.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry rk8Tg; sq9Tg control Protruding-mouth Meckel's cartilage joint IFL
Protruding-mouth palatoquadrate cartilage joint IFL
Protruding-mouth ventral mandibular arch joint IFL
Day 5 Meckel's cartilage joint IFL
Day 5 palatoquadrate cartilage joint IFL
Day 5 ventral mandibular arch joint IFL
rk8Tg; sq9Tg chemical treatment by environment: tricaine methanesulfonate Protruding-mouth Meckel's cartilage joint IFL
Protruding-mouth palatoquadrate cartilage joint IFL
Protruding-mouth ventral mandibular arch joint IFL
Day 5 Meckel's cartilage joint IFL
Day 5 palatoquadrate cartilage joint IFL
Day 5 ventral mandibular arch joint IFL
rk8Tg; sq9Tg + MO1-wnt16 standard conditions Protruding-mouth Meckel's cartilage joint IFL
Protruding-mouth palatoquadrate cartilage joint IFL
Protruding-mouth ventral mandibular arch joint IFL
Day 5 Meckel's cartilage joint IFL
Day 5 palatoquadrate cartilage joint IFL
Day 5 ventral mandibular arch joint IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rk8Tg; sq9Tg chemical treatment by environment: tricaine methanesulfonate Protruding-mouth embryonic skeletal joint development decreased process quality, abnormal
Protruding-mouth joint cell migration decreased occurrence, abnormal
Protruding-mouth joint cell population proliferation decreased occurrence, abnormal
Day 5 embryonic skeletal joint development decreased process quality, abnormal
Day 5 joint cell migration decreased occurrence, abnormal
Day 5 joint cell population proliferation decreased occurrence, abnormal
rk8Tg; sq9Tg + MO1-wnt16 standard conditions Protruding-mouth embryonic skeletal joint development decreased process quality, abnormal
Protruding-mouth joint cell migration decreased occurrence, abnormal
Protruding-mouth joint cell population proliferation decreased occurrence, abnormal
Day 5 embryonic skeletal joint development decreased process quality, abnormal
Day 5 joint cell migration decreased occurrence, abnormal
Day 5 joint cell population proliferation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development