ZFIN ID: ZDB-FIG-170908-30
Brunt et al., 2017 - Wnt signalling controls the response to mechanical loading during Zebrafish joint development. Development (Cambridge, England)   144(15):2798-2809 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 5

Wnt manipulation affects cell proliferation and cell morphology at the jaw joint, revealed using the Zebrabow transgenic line. (A-A″): Tg(ubi:Zebrabow) and Tg(Sox10:cre) transgenic lines generate multiple colours of fluorescence in zebrafish cartilage, including at the region of interest at the retroarticular process (RAP) (white dotted line). Cell outlines were created at the RAP: (A′) RAP cell outlines overlaid with confocal image; (A″) cell outlines. (B,C,D) 3 and 5 dpf Tg(ubi:Zebrabow) and Tg(Sox10:cre) control (B), IWR-1-treated (C) and Wnt16 MO-injected zebrafish (D) (n=10, 11 and 20 joints). The RAP of the MC jaw joint is shown. Asterisks of different colours mark cells at 3 and 5 dpf (indicating re-identification and cell division events). (B′,C′,D′) Outlines of individual cells in the RAP of the MC jaw joint, identified in control (B′), IWR-1-treated (C′) and Wnt16 MO-injected (D′) Tg(ubi:Zebrabow) and Tg(Sox10:cre) transgenic zebrafish. Outline colour of individual cells in B′,C′,D′ matches the asterisk colours in B,C,D.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ba74Tg ; a131Tg chemical treatment by environment: IWR-1-endo Protruding-mouth - Day 5 retroarticular cell population proliferation decreased occurrence, abnormal
Protruding-mouth - Day 5 retroarticular morphology, abnormal
ba74Tg ; a131Tg + MO1-wnt16 standard conditions Protruding-mouth - Day 5 retroarticular cell population proliferation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development