ZFIN ID: ZDB-FIG-170908-28
Brunt et al., 2017 - Wnt signalling controls the response to mechanical loading during Zebrafish joint development. Development (Cambridge, England)   144(15):2798-2809 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 3

Manipulation of Wnt affects zebrafish jaw joint morphology. (A,A′) 5 dpf DMSO control (A) and 5 dpf 20 µM IWR-1-treated (from 3-5 dpf) (A′) zebrafish jaw joint morphology. Left: Tg(Col2a1aBAC:mcherry) transgenic zebrafish line marks the cartilage of the jaw joint. Middle: 3D volume rendering of jaw joint. Right: outlines of four representative jaw joints. A, anterior; P, posterior; M, medial; L, lateral. White lines indicate interzone interval measurements between MC and PQ; yellow line indicates overlapping interval between MC and PQ. (B,B′) Interzone intervals (µm) between the MC and PQ on the medial (B) and lateral (B′) regions of the jaw joint in 5 dpf DMSO and IWR-1-treated zebrafish. Negative values represent an overlap of MC/PQ elements (n=42 and 45 joints). Two-tailed Student's t-tests were performed for B,B′. (C,C′) 5 dpf control-injected (C) and Wnt16 morpholino (MO)-injected (C′) zebrafish jaw joint morphology. Left: immunohistochemical stain of the jaw joint region. Middle: 3D volume rendering of the jaw joint. Right: outlines of four representative jaw joints. (D,D′) Interzone intervals (µm) between the MC and PQ on the medial (D) and lateral (D′) regions of the jaw joint in 5 dpf control-injected and Wnt16 MO-injected zebrafish. Negative values represent an overlap of MC/PQ elements (n=8, 11, 6 and 8 joints). One-way ANOVA calculations were performed (D,D′). ns, not significant, *P≤0.05, ***P≤0.001. Data are mean and 95% confidence interval. (E) 3D volume rendering of 5 dpf injected CRISPR/Cas9 mosaic wnt16 knockout Tg(Col2a1aBAC:mcherry) larvae (1,2) (n=12 animals). Image was enlarged and rotated to best show the jaw joint (1′,2′). Right: outlines of four representative jaw joints.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry hu5910Tg control Day 5 Meckel's cartilage IHC
Day 5 palatoquadrate cartilage IHC
hu5910Tg chemical treatment by environment: IWR-1-endo Day 5 Meckel's cartilage IHC
Day 5 palatoquadrate cartilage IHC
hu5910Tg + CRISPR1-wnt16 + CRISPR2-wnt16 standard conditions Day 5 Meckel's cartilage IHC
Day 5 palatoquadrate cartilage IHC
Day 5 ventral mandibular arch IHC
hu5910Tg + MO1-wnt16 standard conditions Day 5 Meckel's cartilage IHC
Day 5 palatoquadrate cartilage IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hu5910Tg chemical treatment by environment: IWR-1-endo Day 5 Meckel's cartilage joint morphology, abnormal
Day 5 palatoquadrate cartilage joint morphology, abnormal
hu5910Tg + CRISPR1-wnt16 + CRISPR2-wnt16 standard conditions Day 5 Meckel's cartilage joint morphology, abnormal
Day 5 palatoquadrate cartilage joint morphology, abnormal
hu5910Tg + MO1-wnt16 standard conditions Day 5 Meckel's cartilage joint morphology, abnormal
Day 5 palatoquadrate cartilage joint morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development