FIGURE

Fig. S1

ID

ZDB-FIG-170612-4

Publication

Mott et al., 2017 - Expressing acetylcholine receptors after innervation suppresses spontaneous vesicle release and causes muscle fatigue

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Fig. S1

Induction of AChR in muscle cells by RU486. a, The scheme of the DNA construct employed for the RU486-inducible system. α-actin promoter drives the expression of a fusion protein⁴: LexA^DBD+PR^LBD+p65^AD. LexA^DBD is a DNA-binding region of the bacterial LexA protein. PR^LBD is a ligand binding domain of the human progesterone receptor. p65^AD is the activation domain of the human p65. The δ2YFP gene is downstream of the LexA operator fused to the minimal 35S promoter from Cauliflower Mosaic Virus⁴. When RU486 binds to the fusion protein, it binds to the LexA operator and drives the expression of δ2YFP. b, The expression of AChRs induced by the RU-486 application. YFP tagged to the AChR was observed in the trunk muscle region. Scale: 1 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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