PUBLICATION

Expressing acetylcholine receptors after innervation suppresses spontaneous vesicle release and causes muscle fatigue

Authors
Mott, M., Luna, V.M., Park, J.Y., Downes, G.B., Epley, K., Ono, F.
ID
ZDB-PUB-170512-3
Date
2017
Source
Scientific Reports   7: 1674 (Journal)
Registered Authors
Downes, Gerald, Ono, Fumihito, Park, Jee-Young
Keywords
Neurophysiology, Synaptic development, Synaptic vesicle exocytosis
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Locomotion
  • Muscle Fatigue/physiology*
  • Muscle, Skeletal/innervation*
  • Muscle, Skeletal/physiology*
  • Receptors, Cholinergic/metabolism*
  • Synaptic Vesicles/metabolism*
  • Zebrafish
PubMed
28490756 Full text @ Sci. Rep.
Abstract
The formation and function of synapses are tightly orchestrated by the precise timing of expression of specific molecules during development. In this study, we determined how manipulating the timing of expression of postsynaptic acetylcholine receptors (AChRs) impacts presynaptic release by establishing a genetically engineered zebrafish line in which we can freely control the timing of AChR expression in an AChR-less fish background. With the delayed induction of AChR expression after an extensive period of AChR-less development, paralyzed fish displayed a remarkable level of recovery, exhibiting a robust escape response following developmental delay. Despite their apparent behavioral rescue, synapse formation in these fish was significantly altered as a result of delayed AChR expression. Motor neuron innervation determined the sites for AChR clustering, a complete reversal of normal neuromuscular junction (NMJ) development where AChR clustering precedes innervation. Most importantly, among the three modes of presynaptic vesicle release, only the spontaneous release machinery was strongly suppressed in these fish, while evoked vesicle release remained relatively unaffected. Such a specific presynaptic change, which may constitute a part of the compensatory mechanism in response to the absence of postsynaptic AChRs, may underlie symptoms of neuromuscular diseases characterized by reduced AChRs, such as myasthenia gravis.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping