ZFIN ID: ZDB-FIG-170516-43
Goto et al., 2017 - FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step EMT. Development (Cambridge, England)   144(8):1412-1424 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage Range: 14-19 somites to 20-25 somites
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: 20-25 somites

Fig. 6

Wnt promotes EMT initiation and cooperates with FGF to promote EMT termination in tailbud NMPs. (A-F) vimr1 and vimr2 expression was analyzed in 20-somite stage wild-type sibling (A,B), hemizygous HS:TCFΔC (C,D) and homozygous HS:TCFΔC (E,F) embryos. Wnt inhibition leads to the loss of vimr1 and vimr2. (G-J) Expression of vimr1 and vimr2 was also analyzed in HS:caβ-catenin embryos (I,J) and their wild-type siblings (G,H). Wnt overactivation leads to a loss of vimr1 and vimr2 expression and a shift towards the ventral anterior portion of the tailbud (black arrow in I,J). (K-T′) tbx16 and msgn1 expression was examined in control (K-L′,Q-R′), hemizygous HS:TCFΔC (M-N′), homozygous HS:TCFΔC (O-P′) and HS:caβ-catenin (S-T′) embryos. Wnt inhibition had little effect on tbx16 expression but led to decreased expression of msgn1. Wnt overactivation led to expansion of the tbx16 and msgn1 expression domains. (Ua-Xd) Epistasis experiments were performed to examine the control of vimr1 expression by the Wnt and FGF signaling pathways. HS:caβ-catenin embryos were heat shocked at the 12-somite stage and treated with SU5402 or vehicle only (DMSO). These embryos were subsequently fixed 3 h, 4 h, 5 h and 6 h after heat shock induction for in situ hybridization. DMSO-treated control embryos exhibited consistent expression of vimr1 during somitogenesis (Ua-d), whereas DMSO-treated HS:caβ-catenin embryos had decreased expression of the EMT marker in the tailbud (Va-d). Consistent with previous data (Fig. 3), FGF inhibitor-treated embryos had increased expression of vimr1 (Wa-d). HS:caβ-catenin embryos in the presence of SU5402 maintained strong expression of vimr1 (Xa-d). All tailbud images are lateral views, except where indicated otherwise.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
msgn1 WT control 20-25 somites tail bud ISH
w130Tg/w130Tg heat shock 20-25 somites tail bud ISH
w74Tg/+ heat shock 20-25 somites tail bud ISH
w74Tg/w74Tg heat shock 20-25 somites tail bud ISH
tbx16 WT control 20-25 somites tail bud ISH
w130Tg/w130Tg heat shock 20-25 somites tail bud ISH
w74Tg/+ heat shock 20-25 somites tail bud ISH
w74Tg/w74Tg heat shock 20-25 somites tail bud ISH
vimr1 WT control 20-25 somites tail bud ISH
sbu101Tg/sbu101Tg heat shock 20-25 somites tail bud ISH
w130Tg/w130Tg chemical treatment: SU5402 14-19 somites tail bud ISH
20-25 somites tail bud ISH
w130Tg/w130Tg heat shock 20-25 somites tail bud ISH
w130Tg/w130Tg heat shock, chemical treatment: SU5402 14-19 somites tail bud ISH
w74Tg/+ heat shock 20-25 somites tail bud ISH
w74Tg/w74Tg heat shock 20-25 somites Not Detected tail bud ISH
vimr2 WT control 20-25 somites tail bud ISH
w130Tg/w130Tg heat shock 20-25 somites tail bud ISH
w74Tg/+ heat shock 20-25 somites tail bud ISH
w74Tg/w74Tg heat shock 20-25 somites Not Detected tail bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w130Tg/w130Tg heat shock 20-25 somites tail bud vimr1 expression decreased amount, abnormal
20-25 somites tail bud vimr2 expression decreased amount, abnormal
20-25 somites tail bud msgn1 expression increased amount, abnormal
20-25 somites tail bud tbx16 expression increased amount, abnormal
w74Tg/w74Tg heat shock 20-25 somites tail bud vimr2 expression absent, abnormal
20-25 somites tail bud vimr1 expression absent, abnormal
20-25 somites tail bud msgn1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development