ZFIN ID: ZDB-FIG-170516-40
Goto et al., 2017 - FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step EMT. Development (Cambridge, England)   144(8):1412-1424 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 14-19 somites to 20-25 somites
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 14-19 somites to 20-25 somites

Fig. 3

FGF is required for EMT termination during tailbud mesoderm formation. (A-H) The expression of vimr1 and vimr2 was examined at the 20-somite stage in HS:dnfgfr1 embryos (C,D) and their wild-type siblings (A,B) that were heat shocked at the 12-somite stage, as well as in HS:cafgfr1 embryos (G,H) and their wild-type siblings (E,F). Expression of vimr1 and vimr2 is increased in loss of FGF function embryos (C,D, arrowheads) and decreased in gain of FGF function embryos (G,H). (I-P′)The expression of tbx16 and msgn1 decreases in FGF-inhibited tailbuds (K-L′) as compared with sibling controls (I-J′). Conversely, expression of tbx16 and msgn1 increases in FGF-overactivation embryos (O-P′) compared with their wild-type controls (M-N′). White arrowheads (K′,L′; dorsal view) indicate loss of expression at the tip and midline of the tailbud. Black arrowheads (O′,P′; dorsal view) indicate ectopic expression at the tip and midline of the tailbud. (Q-R‴) Co-expression of vimr1 and Tbx16 was examined in 20-somite stage wild-type (Q-Q‴) and HS:dnfgfr1 (R-R‴) embryos that were heat shocked at 12 somites. FGF inhibition increases the expression of vimr1 while reducing the expression domain of Tbx16 (compare Q′,Q″ with R′,R″). All tailbud images are lateral views except where indicated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
msgn1 pd1Tg/+ control 14-19 somites tail bud ISH
pd1Tg/+ heat shock 14-19 somites tail bud ISH
pd3Tg/pd3Tg control 14-19 somites tail bud ISH
pd3Tg/pd3Tg heat shock 14-19 somites tail bud ISH
tbx16 pd1Tg/+ control 14-19 somites tail bud ISH
20-25 somites tail bud IHC
pd1Tg/+ heat shock 14-19 somites tail bud ISH
20-25 somites tail bud IHC
pd3Tg/pd3Tg control 14-19 somites tail bud ISH
pd3Tg/pd3Tg heat shock 14-19 somites tail bud ISH
vimr1 pd1Tg/+ control 14-19 somites notochord ISH
14-19 somites tail bud ISH
20-25 somites notochord ISH
pd1Tg/+ heat shock 14-19 somites notochord ISH
14-19 somites tail bud ISH
20-25 somites notochord ISH
pd3Tg/pd3Tg control 14-19 somites notochord ISH
14-19 somites tail bud ISH
pd3Tg/pd3Tg heat shock 14-19 somites notochord ISH
14-19 somites tail bud ISH
vimr2 pd1Tg/+ control 14-19 somites notochord ISH
14-19 somites tail bud ISH
pd1Tg/+ heat shock 14-19 somites notochord ISH
14-19 somites tail bud ISH
pd3Tg/pd3Tg control 14-19 somites notochord ISH
14-19 somites tail bud ISH
pd3Tg/pd3Tg heat shock 14-19 somites notochord ISH
14-19 somites tail bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pd1Tg/+ heat shock 14-19 somites notochord vimr2 expression increased amount, abnormal
14-19 somites notochord vimr1 expression increased amount, abnormal
14-19 somites tail bud vimr1 expression increased amount, abnormal
14-19 somites tail bud tbx16 expression increased amount, abnormal
14-19 somites tail bud vimr2 expression increased amount, abnormal
14-19 somites tail bud msgn1 expression increased amount, abnormal
20-25 somites notochord vimr1 expression increased amount, abnormal
20-25 somites tail bud tbx16 expression decreased amount, abnormal
pd3Tg/pd3Tg heat shock 14-19 somites notochord vimr2 expression decreased amount, abnormal
14-19 somites notochord vimr1 expression decreased amount, abnormal
14-19 somites tail bud vimr2 expression decreased amount, abnormal
14-19 somites tail bud vimr1 expression decreased amount, abnormal
14-19 somites tail bud tbx16 expression increased amount, abnormal
14-19 somites tail bud msgn1 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development