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ZIRC
ZFIN ID: ZDB-FIG-170516-13
Kärpanen et al., 2017 - An Evolutionarily Conserved Role for Polydom/Svep1 During Lymphatic Vessel Formation. Circulation research   120(8):1263-1275 Full text @ Circ. Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4

Posterior cardinal vein (PCV) cells express pERK and parachordal lymphangioblast (PL) cells express Prox-1 in svep1 mutant embryos. A, Partial maximal projection of antibody staining against Prox-1 (red) and fli1a:GFP (green) in embryos from an svep1+/−; fli1a:GFP incross at 48 h post-fertilization (hpf). Prox-1–positive PL cells are indicated by an arrowhead. B, Quantification of Prox-1–positive PL cells across 9 somites at the horizontal myoseptum (HMS) in siblings (96 out of 106 counted PLs are Prox-1 positive in 18 embryos) and svep1 mutants (28 out of 30 PLs are Prox-1 positive in 8 mutant embryos). C–E, pERK-positive cells were quantified in the PCV by scoring RFP and GFP coexpression (indicated by arrows) laterally across 6 somites in the trunk. C, In ccbe1 morpholino (MO) injected embryos (total number of 6 pERK-positive cells in 5 ccbe1 morphants; total number of 36 pERK-positive cells in 6 uninjected controls) and (D) vegf-chu6410 mutants (total number of 17 pERK-positive cells in 7 mutants and 67 pERK-positive cells in 8 siblings), the amount of pERK-positive cells is significantly reduced, whereas in svep1 mutants (E and F) no difference in pERK can be detected in the PCV (total number of 151 pERK-positive cells in 31 siblings compared with 48 pERK-positive cells in 9 svep1 mutants). F, Partial maximal projections of antibody staining against pERK (red) and fli1a:EGFP (green) in svep1+/−; fli1a:GFP incrosses show no difference in the amount of pERK-positive cells in the PCV at 32 hpf. Bar graphs show mean±SD. For statistical analysis, the Mann–Whitney test was applied in all panels.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab5-prox1 IHC y1Tg standard conditions Long-pec vascular lymphangioblast
IHC y1Tg ; svep1hu4767/hu4767 standard conditions Long-pec vascular lymphangioblast
Ab9-mapk IHC vegfchu6410/hu6410; y1Tg standard conditions Long-pec posterior cardinal vein
IHC y1Tg standard conditions Prim-15 posterior cardinal vein
IHC y1Tg + MO1-ccbe1 standard conditions Long-pec posterior cardinal vein
IHC y1Tg ; svep1hu4767/hu4767 standard conditions Prim-15 posterior cardinal vein
Phenotype Details
Fish Conditions Stage Phenotype
vegfchu6410/hu6410; y1Tg standard conditions Long-pec posterior cardinal vein ab9-mapk labeling decreased distribution, abnormal
y1Tg + MO1-ccbe1 standard conditions Long-pec posterior cardinal vein ab9-mapk labeling decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Circulation research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Circ. Res.