ZFIN ID: ZDB-FIG-170516-10
Kärpanen et al., 2017 - An Evolutionarily Conserved Role for Polydom/Svep1 During Lymphatic Vessel Formation. Circulation research   120(8):1263-1275 Full text @ Circ. Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1

Characterization of a zebrafish mutant that affects the formation of the lymphatic vascular system. A, Gross morphology of the Ly02-512 mutant. The mutant embryos appear without major macroscopic defects or developmental delay, and form a swim bladder at 5 d post-fertilization (dpf). However, closer examination shows abnormalities in the jaw (arrowhead), and the embryo displays edema around the heart, eye, and intestine (arrows). B, Analysis of the vasculature in a fli1a:GFP; flt1enh:RFP transgenic background, highlighting arteries (red). Note the normal overall patterning of the blood vasculature despite the higher proportion of intersegmental arteries in the mutant embryo. C, Mutant embryos lack all or most aspects of the thoracic duct (TD), which in wild-type siblings is positioned just ventral to the dorsal aorta (DA; white arrows in C and D) but is absent in mutants (asterisks). D, Rhodamine dextran injection into the cardinal vein shows normal blood circulation in Ly02-512 mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP hu5333Tg ; y1Tg ; svep1hu4767/hu4767 standard conditions Day 5 endothelial cell IFL
RFP hu5333Tg ; y1Tg ; svep1hu4767/hu4767 standard conditions Day 5 artery IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hu5333Tg ; y1Tg ; svep1hu4767/hu4767 standard conditions Day 5 embryo development ending in birth or egg hatching onset quality, normal
Day 5 intersegmental artery increased amount, abnormal
Day 5 intersegmental vein decreased amount, abnormal
Day 5 lymph vessel development disrupted, abnormal
Day 5 swim bladder present, normal
Day 5 vasculature functional, normal
Day 5 whole organism lacks parts or has fewer parts of type thoracic duct, abnormal
svep1hu4767/hu4767 standard conditions Day 5 brain physical object quality, abnormal
Day 5 embryo development ending in birth or egg hatching onset quality, normal
Day 5 eye edematous, abnormal
Day 5 heart physical object quality, abnormal
Day 5 intestine edematous, abnormal
Day 5 mandibular arch skeleton physical object quality, abnormal
Day 5 pericardium edematous, abnormal
Day 5 swim bladder present, normal
Acknowledgments:
ZFIN wishes to thank the journal Circulation research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Circ. Res.