ZFIN ID: ZDB-FIG-170420-10 |
Genes: | |
---|---|
Fish: | |
Anatomical Terms: | |
Stage Range: | 10-13 somites to 26+ somites |
Fish: | |
---|---|
Observed In: | |
Stage Range: | 20-25 somites to 26+ somites |
Fig. 3 vegfaa mutants display defects in cardiomyocytes fusion. (A–D) In situ hybridization depicts myl7 expression at 21- and 26-somite stage in wild-type embryos (A,C) and vegfaa mutant embryos (B,D). At 21-somite stage, the wild-type cardiomyocytes formed a ring (A), whereas few cardiomyocytes are formed in the vegfaa mutants (B). At 26-somite stage, the wild-type primitive heart tube begins left looping (C) while vegfaa mutant embryos are still cardiac cone (D); (E,F) In situ hybridization depicts ventricular myosin heavy chain (vmhc) expression at 20-somite stage in wild-type embryos (E) and vegfaa mutant embryos (F); (G–J) Dorsal views depict unaffected endocardial expression pattern of kdrl in vegfaa mutants. Black arrows indicate midline migration of endocardial cells; Scale bar: 200 µm (A–J); (K,L) Dorsal views depict endocardial expression of Tg(kdrl:EGFP) at the 22-somite stage. White arrows indicate leftward movement. Scale bar: 50 µm. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
---|---|---|---|---|---|---|
GFP | la116Tg | control | 20-25 somites | endocardium | IFL | |
20-25 somites | endocardium endothelial cell | IFL | ||||
la116Tg ; vegfaazf1090/zf1090 | control | 20-25 somites | endocardium | IFL | ||
20-25 somites | endocardium endothelial cell | IFL | ||||
kdrl | AB | control | 10-13 somites | endocardium | ISH | |
10-13 somites | endocardium endothelial cell | ISH | ||||
14-19 somites | endocardium | ISH | ||||
14-19 somites | endocardium endothelial cell | ISH | ||||
vegfaazf1090/zf1090 | control | 10-13 somites | endocardium | ISH | ||
10-13 somites | endocardium endothelial cell | ISH | ||||
14-19 somites | endocardium | ISH | ||||
14-19 somites | endocardium endothelial cell | ISH | ||||
myh7 | AB | control | 20-25 somites | cardiac muscle cell | ISH | |
vegfaazf1090/zf1090 | control | 20-25 somites | cardiac muscle cell | ISH | ||
myl7 | AB | control | 20-25 somites | cardiac muscle cell | ISH | |
26+ somites | cardiac muscle cell | ISH | ||||
vegfaazf1090/zf1090 | control | 20-25 somites | cardiac muscle cell | ISH | ||
26+ somites | cardiac muscle cell | ISH |
Acknowledgments: | ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci. |
---|