ZFIN ID: ZDB-FIG-170420-10
Zhu et al., 2017 - Vegfa Impacts Early Myocardium Development in Zebrafish. International Journal of Molecular Sciences   18(2) Full text @ Int. J. Mol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to 26+ somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to 26+ somites

Fig. 3

vegfaa mutants display defects in cardiomyocytes fusion. (A–D) In situ hybridization depicts myl7 expression at 21- and 26-somite stage in wild-type embryos (A,C) and vegfaa mutant embryos (B,D). At 21-somite stage, the wild-type cardiomyocytes formed a ring (A), whereas few cardiomyocytes are formed in the vegfaa mutants (B). At 26-somite stage, the wild-type primitive heart tube begins left looping (C) while vegfaa mutant embryos are still cardiac cone (D); (E,F) In situ hybridization depicts ventricular myosin heavy chain (vmhc) expression at 20-somite stage in wild-type embryos (E) and vegfaa mutant embryos (F); (G–J) Dorsal views depict unaffected endocardial expression pattern of kdrl in vegfaa mutants. Black arrows indicate midline migration of endocardial cells; Scale bar: 200 µm (A–J); (K,L) Dorsal views depict endocardial expression of Tg(kdrl:EGFP) at the 22-somite stage. White arrows indicate leftward movement. Scale bar: 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP la116Tg control 20-25 somites endocardium IFL
20-25 somites endocardium endothelial cell IFL
la116Tg ; vegfaazf1090/zf1090 control 20-25 somites endocardium IFL
20-25 somites endocardium endothelial cell IFL
kdrl AB control 10-13 somites endocardium ISH
10-13 somites endocardium endothelial cell ISH
14-19 somites endocardium ISH
14-19 somites endocardium endothelial cell ISH
vegfaazf1090/zf1090 control 10-13 somites endocardium ISH
10-13 somites endocardium endothelial cell ISH
14-19 somites endocardium ISH
14-19 somites endocardium endothelial cell ISH
myh7 AB control 20-25 somites cardiac muscle cell ISH
vegfaazf1090/zf1090 control 20-25 somites cardiac muscle cell ISH
myl7 AB control 20-25 somites cardiac muscle cell ISH
26+ somites cardiac muscle cell ISH
vegfaazf1090/zf1090 control 20-25 somites cardiac muscle cell ISH
26+ somites cardiac muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
vegfaazf1090/zf1090 control 20-25 somites presumptive cardiac ventricle primitive heart tube cardioblast migration to the midline involved in heart field formation delayed, abnormal
20-25 somites - 26+ somites cardioblast migration to the midline involved in heart field formation delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.