ZFIN ID: ZDB-PUB-170224-3
Vegfa Impacts Early Myocardium Development in Zebrafish
Zhu, D., Fang, Y., Gao, K., Shen, J., Zhong, T.P., Li, F.
Date: 2017
Source: International Journal of Molecular Sciences   18(2): (Journal)
Registered Authors: Zhong, Tao P.
Keywords: Vegfa, cardiac fusion, zebrafish
MeSH Terms:
  • Animals
  • Cell Proliferation
  • Gene Expression Regulation, Developmental
  • Heart/embryology*
  • Morphogenesis/genetics
  • Mutation
  • Myocardium/metabolism*
  • Myocytes, Cardiac/metabolism
  • Organogenesis/genetics*
  • Phenotype
  • Vascular Endothelial Growth Factor A/deficiency
  • Vascular Endothelial Growth Factor A/genetics*
  • Vascular Endothelial Growth Factor A/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed: 28230770 Full text @ Int. J. Mol. Sci.
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ABSTRACT
Vascular endothelial growth factor A (Vegfa) signaling regulates cardiovascular development. However, the cellular mechanisms of Vegfa signaling in early cardiogenesis remain poorly understood. The present study aimed to understand the differential functions and mechanisms of Vegfa signaling in cardiac development. A loss-of-function approach was utilized to study the effect of Vegfa signaling in cardiogenesis. Both morphants and mutants for vegfaa display defects in cardiac looping and chamber formation, especially the ventricle. Vegfa regulates the heart morphogenesis in a dose-dependent manner. Furthermore, the initial fusion of the bilateral myocardium population is delayed rather than endocardium. The results demonstrate that Vegfa signaling plays a direct impact on myocardium fusion, indicating that it is the initial cause of the heart defects. The heart morphogenesis is regulated by Vegfa in a dose-dependent manner, and later endocardium defects may be secondary to impaired myocardium-endocardium crosstalk.
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