ZFIN ID: ZDB-FIG-161230-4
Nichols et al., 2016 - Ligament versus bone cell identity in the zebrafish hyoid skeleton is regulated by mef2ca. Development (Cambridge, England)   143(23):4430-4440 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 2

The orthogonal tissue track has ligament identity, which is lost when ectopic bone develops in mef2cab1086. (A,D) Confocal images of sp7 (green) and scxa (red) transcripts detected by in situ hybridization in 5 dpf zebrafish. (B,E) Confocal images of 5 dpf sp7:EGFP (green) transgenic larvae immunostained with anti-Thbs4b antibody (red). (C,F) Confocal images of live 6 dpf sp7:EGFP (green) and alx4a:DsRed2 (red) transgenic larvae. Wild-type (WT) and mutant (mef2cab1086) images were uniformly adjusted for brightness and contrast. The opercle (op) and branchiostegal ray (br) are indicated. Arrows mark putative ligaments, arrowheads denote tendons, asterisks mark ectopic bone where ligament identity is lost. All images are projections of several confocal sections. Lateral views, anterior is towards the left and dorsal is up. Scale bars: 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 b1212Tg ; mef2cab1086/b1086 ; pd52Tg standard conditions Day 5 ligament IFL
pd52Tg ; b1212Tg standard conditions Day 5 ligament IFL
Day 5 operculohyoid ligament IFL
EGFP b1212Tg standard conditions Day 5 branchiostegal ray osteoblast IFL
Day 5 opercle osteoblast IFL
b1212Tg ; mef2cab1086/b1086 ; pd52Tg standard conditions Day 5 dermal bone osteoblast IFL
mef2cab1086/b1086 ; b1212Tg standard conditions Day 5 dermal bone osteoblast IFL
pd52Tg ; b1212Tg standard conditions Day 5 branchiostegal ray osteoblast IFL
Day 5 opercle osteoblast IFL
scxa WT standard conditions Day 5 operculohyoid ligament ISH
mef2cab1086/b1086 standard conditions Day 5 ligament ISH
sp7 WT standard conditions Day 5 branchiostegal ray osteoblast ISH
Day 5 opercle osteoblast ISH
mef2cab1086/b1086 standard conditions Day 5 branchiostegal ray osteoblast ISH
Day 5 opercle osteoblast ISH
Day 5 operculohyoid ligament osteoblast ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-thbs4b IHC b1212Tg standard conditions Day 5 operculohyoid ligament extracellular matrix
IHC Day 5 tendon
IHC mef2cab1086/b1086 ; b1212Tg standard conditions Day 5 ligament
IHC Day 5 tendon
Phenotype Details
Fish Conditions Stage Phenotype
b1212Tg ; mef2cab1086/b1086 ; pd52Tg standard conditions Day 5 branchiostegal ray fused with opercle, abnormal
Day 5 branchiostegal ray increased size, abnormal
Day 5 ligamentous ossification increased occurrence, abnormal
Day 5 opercle increased size, abnormal
Day 5 operculohyoid ligament ossified, abnormal
mef2cab1086/b1086 standard conditions Day 5 branchiostegal ray fused with opercle, abnormal
Day 5 branchiostegal ray increased size, abnormal
Day 5 head has extra parts of type bone tissue, abnormal
Day 5 opercle increased size, abnormal
Day 5 operculohyoid ligament osteoblast sp7 expression mislocalised, abnormal
mef2cab1086/b1086 ; b1212Tg standard conditions Day 5 branchiostegal ray fused with opercle, abnormal
Day 5 branchiostegal ray increased size, abnormal
Day 5 head has extra parts of type bone tissue, abnormal
Day 5 ligamentous ossification increased occurrence, abnormal
Day 5 opercle increased size, abnormal
Day 5 operculohyoid ligament ossified, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development