ZFIN ID: ZDB-FIG-161230-3
Nichols et al., 2016 - Ligament versus bone cell identity in the zebrafish hyoid skeleton is regulated by mef2ca. Development (Cambridge, England)   143(23):4430-4440 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

Ectopic bone cells appear in place of ligament cells in mef2cab1086. (A,B) Excerpts from a time-lapse movie imaging sp7:EGFP (green) and alx4a:DsRed2 (red) transgenic larvae. Arrows mark ligament cells, asterisks label ectopic bone cells. Background green fluorescence of the skin is visible in some images. Lateral views, anterior is towards the left and dorsal is up. op, opercle; WT, wild type. Scale bar: 10 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 b1212Tg ; mef2cab1086/b1086 ; pd52Tg standard conditions Long-pec portion of connective tissue IFL
Pec-fin portion of connective tissue IFL
Protruding-mouth portion of connective tissue IFL
pd52Tg ; b1212Tg standard conditions Long-pec portion of connective tissue IFL
Pec-fin ligament IFL
Pec-fin operculohyoid ligament IFL
Pec-fin tendon IFL
Protruding-mouth ligament IFL
Protruding-mouth operculohyoid ligament IFL
Protruding-mouth tendon IFL
EGFP b1212Tg ; mef2cab1086/b1086 ; pd52Tg standard conditions Long-pec opercle osteoblast IFL
Pec-fin branchiostegal ray osteoblast IFL
Pec-fin opercle osteoblast IFL
Pec-fin operculohyoid ligament osteoblast IFL
Protruding-mouth branchiostegal ray osteoblast IFL
Protruding-mouth opercle osteoblast IFL
Protruding-mouth operculohyoid ligament osteoblast IFL
pd52Tg ; b1212Tg standard conditions Long-pec opercle osteoblast IFL
Pec-fin opercle osteoblast IFL
Protruding-mouth opercle osteoblast IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
b1212Tg ; mef2cab1086/b1086 ; pd52Tg standard conditions Long-pec portion of connective tissue DsRed2 expression decreased amount, abnormal
Pec-fin ligamentous ossification increased occurrence, abnormal
Pec-fin operculohyoid ligament osteoblast EGFP expression mislocalised, abnormal
Pec-fin portion of connective tissue DsRed2 expression decreased amount, abnormal
Protruding-mouth ligamentous ossification increased occurrence, abnormal
Protruding-mouth operculohyoid ligament osteoblast EGFP expression mislocalised, abnormal
Protruding-mouth portion of connective tissue DsRed2 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development