ZFIN ID: ZDB-FIG-161230-1
Nichols et al., 2016 - Ligament versus bone cell identity in the zebrafish hyoid skeleton is regulated by mef2ca. Development (Cambridge, England)   143(23):4430-4440 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Day 6
PHENOTYPE:
Fish:
Observed In:
Stage: Day 6

Fig. 1

mef2cab1086 bone phenotypes and skeletal motion suggest a unique track of tissue orthogonal to the opercle. (A,B) Confocal projections of osteoblasts (sp7:EGFP) and bones (Alizarin Red) from live 6 dpf zebrafish. In A, the opercle (op) branchiostegal ray (br), cleithrum (c) and ceratobranchial 5 (cb5) bony elements are labeled and the eye, ear and mouth (m) are indicated for reference. In B, op and br bone phenotypes in wild types (WT), mef2cab1086 mutants with bone phenotypes resembling wild type (mef2cab1086 mild), and mutants with bony expansions (mef2cab1086 severe) are shown in this composite image. Arrows indicate a narrow track of extra bone. Images are projections of several confocal sections. (C) ‘Skeletonized’ time-lapse movies tracking the location and direction of ectopic growth (arrows). The major direction of op growth is along the y-axis. (D) Overlays of skeletonized time-lapse recordings from wild types and mutants. (E) Excerpts from a movie in which the head skeleton cartilage (sox9a:EGFP) and bone (sp7:EGFP) were imaged in the same fluorescent channel from a non-anesthetized larva and outlined (red). The same animal was imaged 0.1 s later and the head skeleton was outlined again (green) and overlaid on the first image revealing skeletal motion. All images are lateral views, anterior is towards the left and dorsal is upward in all panels. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP b1212Tg standard conditions Day 6 branchiostegal ray osteoblast IFL
Day 6 ceratobranchial 5 bone osteoblast IFL
Day 6 cleithrum osteoblast IFL
Day 6 mandibular arch skeleton osteoblast IFL
mef2cab1086/b1086 ; b1212Tg standard conditions Day 6 branchiostegal ray osteoblast IFL
Day 6 ceratobranchial 5 bone osteoblast IFL
Day 6 cleithrum osteoblast IFL
Day 6 dermal bone osteoblast IFL
Day 6 mandibular arch skeleton osteoblast IFL
Day 6 replacement bone osteoblast IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mef2cab1086/b1086 ; b1212Tg standard conditions Day 6 branchiostegal ray fused with opercle, abnormal
Day 6 branchiostegal ray increased size, abnormal
Day 6 head has extra parts of type bone tissue, abnormal
Day 6 opercle increased size, abnormal
Day 6 replacement bone osteoblast EGFP expression mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development