FIGURE

Fig. 5

ID

ZDB-FIG-161229-10

Publication

James et al., 2016 - The Hyaloid Vasculature Facilitates Basement Membrane Breakdown During Choroid Fissure Closure in the Zebrafish Eye

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Fig. 5

talin1 is required for CFC in zebrafish. (A) talin1 is expressed within the POM and retinal/RPE cells lining the CF at 33 hpf (arrow). (B,C) Lateral views of the eye of tln1^hi3093Tg mutant (C) and wild-type sibling (B) at 3dpf. tln1 mutants possess colobomas. (d-F) Distal section through the eye of a 32 hpf embryo demonstrating talin1 expression within the retina/RPE cells lining the CF (arrows) and the hyaloid vasculature (marked by GFP expression from fli1a:eGFP; arrowhead). (G-J) Sagittal sections through the eyes of 48 hpf tln1 mutants and siblings stained with Lam-111 (red) and Sytox-green (DNA; green). BM degradation is disrupted in tln1 mutants at 48 hpf. (K-N) Maximum projection images of the distal hyaloid in tln1 mutants and siblings demonstrating severe hypotrophy of the hyaloid in the tln1 mutant at 44.5 hpf. Scale bar=20 µm (G-J, K,M) and 50 µm (L,N).

Expression Data

Genes:	mCherry tln1
Antibodies:	Ab1-lama1 Ab4-GFP
Fish:	WT tln1^{hi3093Tg/hi3093Tg} is5Tg y1Tg tln1^{hi3093Tg/hi3093Tg}; is5Tg
Anatomical Terms:	hyaloid vessel optic furrow optic furrow basement membrane periocular mesenchyme retina retinal pigmented epithelium
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tln1^{hi3093Tg/hi3093Tg} tln1^{hi3093Tg/hi3093Tg}; is5Tg
Observed In:	closure of optic fissure hyaloid vessel optic fissure optic furrow basement membrane optic furrow basement membrane disassembly
Stage Range:	High-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 419(2), James, A., Lee, C., Williams, A.M., Angileri, K., Lathrop, K.L., Gross, J.M., The Hyaloid Vasculature Facilitates Basement Membrane Breakdown During Choroid Fissure Closure in the Zebrafish Eye, 262-272, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.