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ZFIN ID: ZDB-FIG-161229-10
James et al., 2016 - The Hyaloid Vasculature Facilitates Basement Membrane Breakdown During Choroid Fissure Closure in the Zebrafish Eye. Developmental Biology   419(2):262-272 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: High-pec to Protruding-mouth

Fig. 5

talin1 is required for CFC in zebrafish. (A) talin1 is expressed within the POM and retinal/RPE cells lining the CF at 33 hpf (arrow). (B,C) Lateral views of the eye of tln1hi3093Tg mutant (C) and wild-type sibling (B) at 3dpf. tln1 mutants possess colobomas. (d-F) Distal section through the eye of a 32 hpf embryo demonstrating talin1 expression within the retina/RPE cells lining the CF (arrows) and the hyaloid vasculature (marked by GFP expression from fli1a:eGFP; arrowhead). (G-J) Sagittal sections through the eyes of 48 hpf tln1 mutants and siblings stained with Lam-111 (red) and Sytox-green (DNA; green). BM degradation is disrupted in tln1 mutants at 48 hpf. (K-N) Maximum projection images of the distal hyaloid in tln1 mutants and siblings demonstrating severe hypotrophy of the hyaloid in the tln1 mutant at 44.5 hpf. Scale bar=20 µm (G-J, K,M) and 50 µm (L,N).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry is5Tg standard conditions High-pec hyaloid vessel IFL
tln1hi3093Tg/hi3093Tg ; is5Tg standard conditions High-pec hyaloid vessel IFL
tln1 WT standard conditions Prim-15 optic furrow ISH
Prim-15 periocular mesenchyme ISH
Prim-15 retina ISH
Prim-15 retinal pigmented epithelium ISH
y1Tg standard conditions Prim-15 optic furrow ISH
Prim-15 periocular mesenchyme ISH
Prim-15 retina ISH
Prim-15 retinal pigmented epithelium ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-lama1 IHC WT standard conditions Long-pec Not Detected optic furrow
IHC tln1hi3093Tg/hi3093Tg standard conditions Long-pec optic furrow basement membrane
Ab4-GFP IHC y1Tg standard conditions Prim-15 hyaloid vessel
Phenotype Details
Fish Conditions Stage Phenotype
tln1hi3093Tg/hi3093Tg standard conditions Long-pec optic furrow basement membrane ab1-lama1 labeling increased distribution, abnormal
Long-pec optic furrow basement membrane present, abnormal
Long-pec optic furrow basement membrane disassembly disrupted, abnormal
Protruding-mouth closure of optic fissure disrupted, abnormal
Protruding-mouth optic fissure closure incomplete, abnormal
tln1hi3093Tg/hi3093Tg ; is5Tg standard conditions High-pec hyaloid vessel mCherry expression decreased distribution, abnormal
High-pec hyaloid vessel hypotrophic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 419(2), James, A., Lee, C., Williams, A.M., Angileri, K., Lathrop, K.L., Gross, J.M., The Hyaloid Vasculature Facilitates Basement Membrane Breakdown During Choroid Fissure Closure in the Zebrafish Eye, 262-272, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.