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ZFIN ID: ZDB-FIG-161229-8
James et al., 2016 - The Hyaloid Vasculature Facilitates Basement Membrane Breakdown During Choroid Fissure Closure in the Zebrafish Eye. Developmental Biology   419(2):262-272 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Prim-25

Fig. 4

Periocular mesenchymal cells contribute to CFC. (A-C) Sagittal views of the CF stained with anti-GFP (green), Lam-111 (red) and/or phalloidin (blue). (A) Few sox10:eGFP+ cells are detected in the CF, 37 hpf section pictured. (B) fli1a:eGFP+ cells are retained in the CF. 36 hpf section pictured. (C) fli1a:eGFP+ cells possess F-actin accumulations that localize to regions of BM breakdown. Arrows denote puncta of F-actin where Lam-111 is low or absent. 34 hpf section pictured. Scale bar=20 µm (A,B) and 10 µm (C).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-lama1 IHC ba2Tg standard conditions Prim-25 optic furrow basement membrane
IHC y1Tg standard conditions Prim-15 optic furrow basement membrane
IHC Prim-25 optic furrow basement membrane
Ab4-GFP IHC ba2Tg standard conditions Prim-25 optic furrow
IHC y1Tg standard conditions Prim-15 optic furrow
IHC Prim-25 optic furrow
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 419(2), James, A., Lee, C., Williams, A.M., Angileri, K., Lathrop, K.L., Gross, J.M., The Hyaloid Vasculature Facilitates Basement Membrane Breakdown During Choroid Fissure Closure in the Zebrafish Eye, 262-272, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.