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ZFIN ID: ZDB-FIG-161201-19
Shin et al., 2016 - Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors. Development (Cambridge, England)   143(20):3785-3795 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 7

Signaling through Flt4 Y1226/7 is dispensable for facial lymphatic formation and function. (A-F) Confocal images of Tg(fli1a:egfp)y1 embryos at 36 hpf of the indicated genotype immunostained with antibodies against Prox1 in red and GFP in green. (A-C) Arrowheads denote Prox1-positive endothelial cells in facial lymphatic sprout (FLS) emerging from the common cardinal vein (CCV, indicated by an arrow). (D-F) Prox1-positive endothelial cells within primary head sinus (PHS, indicated by an arrow) at 36 hpf denoted by arrowheads. (G) Quantification of Prox1-positive cells from the CCV at 36 hpf; ns, not statistically significant; error bars are ±s.d. (H-K) Confocal images of live Tg(lyve1b:dsRed2)nz101 embryos of the indicated genotype at 4 dpf; pseudocoloring is based on depth: red is surface, green and blue are deeper. Lateral facial lymphatic (LFL), otolithic lymphatic vessel (OLV), posterior cerebral vein (PCeV) and forming lymphatic aortic arches (LAA) are indicated. Arrowhead denotes dorsal wall of the primary head sinus (PHS), which is pseudocolored green. Arrows indicated by asterisks denote stunted LFL. (A-F,H-K) Lateral views, anterior to the left, dorsal is up. (L) Percentage of embryos of the indicated genotype with FLV formation. Numbers of embryos scored in each category are indicated; ***P<0.001; ns, not significant. (M-Q) Dorsal views, anterior to the left. (M-M‴) Live wild-type Tg(fli1a:egfp)y1;(gata1:dsRed2)sd2 embryo at 4 dpf subjected to microangiography with Qdots655 (gray). Arrows denote Qdot perfusion of FLV, bracket indicates PHS lumen. (N-Q) Confocal images of live Tg(fli1a:egfp)y1 embryos at 4 dpf of the indicated genotype subjected to microangiography with Qdots655 (red). Arrows denote FLV, bracket indicates the lumen of the PHS.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed flt4um131/um131 ; nz101Tg standard conditions Day 4 lateral facial lymph vessel IFL
Day 4 Not Detected lateral facial lymph vessel lymphangiogenic sprout IFL
Day 4 Not Detected otolithic lymph vessel IFL
Day 4 posterior cerebral vein IFL
Day 4 primary head sinus IFL
nz101Tg standard conditions Day 4 lateral facial lymph vessel IFL
Day 4 lateral facial lymph vessel lymphangiogenic sprout IFL
Day 4 otolithic lymph vessel IFL
Day 4 posterior cerebral vein IFL
Day 4 primary head sinus IFL
nz101Tg ; flt4um200/um200 standard conditions Day 4 lateral facial lymph vessel IFL
Day 4 lateral facial lymph vessel lymphangiogenic sprout IFL
Day 4 Not Detected otolithic lymph vessel IFL
Day 4 posterior cerebral vein IFL
Day 4 primary head sinus IFL
sd2Tg; y1Tg standard conditions Day 4 nucleate erythrocyte IFL
Day 4 primary head sinus nucleate erythrocyte IFL
vegfcum18/um18 ; nz101Tg standard conditions Day 4 lateral facial lymph vessel IFL
Day 4 Not Detected lateral facial lymph vessel lymphangiogenic sprout IFL
Day 4 Not Detected otolithic lymph vessel IFL
Day 4 posterior cerebral vein IFL
Day 4 primary head sinus IFL
EGFP flt4um131/um131 ; y1Tg standard conditions Prim-25 common cardinal vein IHC
Prim-25 cranial blood vessel endothelial cell IHC
Day 4 lateral facial lymph vessel IFL
sd2Tg; y1Tg standard conditions Day 4 lateral facial lymph vessel IFL
vegfcum18/um18 ; y1Tg standard conditions Day 4 lateral facial lymph vessel IFL
y1Tg standard conditions Prim-25 common cardinal vein IHC
Prim-25 cranial blood vessel endothelial cell IHC
Day 4 lateral facial lymph vessel IFL
y1Tg ; flt4um200/um200 standard conditions Prim-25 common cardinal vein IHC
Prim-25 cranial blood vessel endothelial cell IHC
Day 4 lateral facial lymph vessel IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab10-prox1 IHC y1Tg standard conditions Prim-25 common cardinal vein facial lymphatic sprout
IHC Prim-25 cranial blood vessel endothelial cell
IHC y1Tg ; flt4um131/+ standard conditions Prim-25 common cardinal vein facial lymphatic sprout
IHC Prim-25 cranial blood vessel endothelial cell
IHC y1Tg ; flt4um200/um200 standard conditions Prim-25 common cardinal vein facial lymphatic sprout
IHC Prim-25 cranial blood vessel endothelial cell
Phenotype Details
Fish Conditions Stage Phenotype
flt4um131/um131 ; nz101Tg standard conditions Day 4 lateral facial lymph vessel truncated, abnormal
Day 4 lateral facial lymph vessel lymphangiogenic sprout DsRed expression absent, abnormal
Day 4 lateral facial lymph vessel lymphangiogenic sprout absent, abnormal
Day 4 otolithic lymph vessel absent, abnormal
Day 4 otolithic lymph vessel DsRed expression absent, abnormal
flt4um131/um131 ; y1Tg standard conditions Day 4 lateral facial lymph vessel closed, abnormal
Day 4 lateral facial lymph vessel malformed, abnormal
Day 4 lateral facial lymph vessel lymph vessel development process quality, abnormal
nz101Tg ; flt4um200/um200 standard conditions Day 4 otolithic lymph vessel DsRed expression absent, abnormal
Day 4 otolithic lymph vessel lymph vessel development delayed, abnormal
vegfcum18/um18 ; nz101Tg standard conditions Day 4 lateral facial lymph vessel truncated, abnormal
Day 4 lateral facial lymph vessel lymphangiogenic sprout absent, abnormal
Day 4 lateral facial lymph vessel lymphangiogenic sprout DsRed expression absent, abnormal
Day 4 otolithic lymph vessel absent, abnormal
Day 4 otolithic lymph vessel DsRed expression absent, abnormal
vegfcum18/um18 ; y1Tg standard conditions Day 4 lateral facial lymph vessel closed, abnormal
Day 4 lateral facial lymph vessel malformed, abnormal
Day 4 lateral facial lymph vessel lymph vessel development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development