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ZFIN ID: ZDB-FIG-161201-16
Shin et al., 2016 - Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors. Development (Cambridge, England)   143(20):3785-3795 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 4

ERK signaling is essential for trunk lymphatic morphogenesis and differentiation. (A-I) Confocal images; lateral views, anterior to the left, dorsal is up. (A,B) pERK (red) and GFP (green) immunostaining in Tg(fli1a:egfp)y1 embryos of the indicated genotype at 34 hpf. Arrows denote pERK-positive lympho-venous sprouts, arrowheads indicate pERK staining in motor neurons. Brackets indicate DA and PCV. (C) Prox1 (red) and pERK (green) immunostaining in a wild-type embryo at 38 hpf. Arrows denote endothelial cells that are positive for Prox1 and pERK in lymphatic sprouts or dorsal wall of PCV. (D-I) Embryos treated with DMSO (D-F) or 15 µM SL327 (G-I). Treatment time is indicated. (D,G) Tie2 immunostaining; arrows denote lympho-venous sprouts, PCV indicated by bracket. (E,H) Prox1 (red) and GFP (green) immunostaining of Tg(fli1a:egfp)y1 embryos at 36 hpf; arrows denote Prox1-positive lymphatic sprouts, arrowheads indicate muscle pioneers and Corpuscle of Stannius (cs) is indicated. (F,I) Live Tg(fli1a:egfp)y1 embryos at 49 hpf. Arrows indicate parachordal cells, or absence thereof.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Prim-25 endothelial cell IHC
Long-pec endothelial cell IFL
y1Tg standard conditions Prim-15 dorsal aorta IHC
Prim-15 posterior cardinal vein IHC
y1Tg chemical treatment by environment: SL-327 Prim-25 endothelial cell IHC
Long-pec endothelial cell IFL
y1Tg ; flt4um200/um200 standard conditions Prim-15 dorsal aorta IHC
Prim-15 posterior cardinal vein IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-tek IHC WT control Prim-25 posterior cardinal vein vascular sprouts
IHC Prim-25 vascular sprouts
IHC WT chemical treatment by environment: SL-327 Prim-25 Not Detected posterior cardinal vein vascular sprouts
IHC Prim-25 Not Detected vascular sprouts
Ab9-mapk IHC WT standard conditions Prim-25 lymphangiogenic sprout
IHC Prim-25 posterior cardinal vein endothelial cell
IHC y1Tg standard conditions Prim-15 motor neuron
IHC Prim-15 posterior cardinal vein endothelial cell
IHC Prim-15 posterior cardinal vein vascular sprouts
IHC Prim-15 vascular sprouts
IHC y1Tg ; flt4um200/um200 standard conditions Prim-15 motor neuron
IHC Prim-15 Not Detected posterior cardinal vein endothelial cell
IHC Prim-15 Not Detected posterior cardinal vein vascular sprouts
IHC Prim-15 vascular sprouts
Ab10-prox1 IHC WT standard conditions Prim-25 lymphangiogenic sprout
IHC Prim-25 posterior cardinal vein endothelial cell
IHC y1Tg control Prim-25 corpuscles of Stannius
IHC Prim-25 lymphangiogenic sprout
IHC Prim-25 muscle pioneer
IHC y1Tg chemical treatment by environment: SL-327 Prim-25 corpuscles of Stannius
IHC Prim-25 Not Detected lymphangiogenic sprout
IHC Prim-25 muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: SL-327 Prim-25 lymphangiogenic sprout absent, abnormal
Prim-25 posterior cardinal vein vascular sprouts Ab2-tek labeling absent, abnormal
Prim-25 posterior caudal vein vascular sprouts absent, abnormal
Prim-25 vascular sprouts Ab2-tek labeling absent, abnormal
y1Tg chemical treatment by environment: SL-327 Prim-25 lymphangiogenic sprout absent, abnormal
Prim-25 lymphangiogenic sprout Ab10-prox1 labeling absent, abnormal
Prim-25 posterior caudal vein vascular sprouts absent, abnormal
Long-pec parachordal vessel absent, abnormal
Long-pec parachordal vessel vasculature development decreased occurrence, abnormal
y1Tg ; flt4um200/um200 standard conditions Prim-15 posterior cardinal vein endothelial cell ab9-mapk labeling absent, abnormal
Prim-15 posterior cardinal vein vascular sprouts ab9-mapk labeling absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development