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ZIRC
ZFIN ID: ZDB-FIG-161201-15
Shin et al., 2016 - Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors. Development (Cambridge, England)   143(20):3785-3795 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. 3

Signaling through Y1226/7 is essential for initial lympho-venous sprouting and lymphatic differentiation. (A-C) Confocal microscopy of Tie2 immunostaining at 36 hpf. Arrows denote lympho-venous sprouts which give rise to trunk lymphatic and intersomitic vein (ISV) in wild-type embryos; arrowheads indicate intersomitic arteries (ISAs), which display Tie2 faint immunostaining in all embryos. Bracket indicates PCV. (D) Percentage of ISA or ISV connections at 72 hpf in embryos of the indicated genotype as determined by angiography. Total numbers of vessels counted for analysis are indicated. (E-G) Confocal microscopy of live Tg(fli1a:egfp)y1 embryos of the indicated genotype at 48 hpf. White arrows denote parachordal cells (PACs) in wild-type embryos. Blue and red arrowheads indicate ISVs and ISAs, respectively. Asterisks denote normal position of PACs; occasional fluorescence is observed in non-endothelial cells at the horizontal myoseptum (e.g. panel F). (H,I) Confocal images of Prox1 (red) and GFP (green) immunostaining in Tg(fli1a:egfp)y1 embryos of the indicated genotype at 36 hpf. Arrows denote Prox1-expressing endothelial cells in lymphatic sprouts; Corpuscle of Stannius (cs) is indicated and asterisk denotes Prox1-positive muscle pioneer. (J,K) Average number of Prox1-positive venous endothelial cells and lymphatic sprouts per embryo of the indicated genotype and at 36 hpf (J) or the indicated stage (K). Total number of embryos scored in each category is indicated on the x-axis. (D,J,K) **P<0.01, ***P<0.001; ns, not significant. Error bars are ±s.d. (A-C,E-I) Lateral views, anterior to the left, dorsal is up. Genotype is indicated for each panel.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP flt4um131/um131 ; y1Tg standard conditions Long-pec dorsal aorta IFL
Long-pec intersegmental artery IFL
Long-pec Not Detected intersegmental vein IFL
y1Tg standard conditions Prim-25 posterior cardinal vein IHC
Long-pec dorsal aorta IFL
Long-pec intersegmental artery IFL
Long-pec intersegmental vein IFL
y1Tg ; flt4um200/um200 standard conditions Prim-25 posterior cardinal vein IHC
Long-pec dorsal aorta IFL
Long-pec intersegmental artery IFL
Long-pec Not Detected intersegmental vein IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-tek IHC WT standard conditions Prim-25 posterior cardinal vein vascular sprouts
IHC Prim-25 vascular sprouts
IHC flt4um131/um131 standard conditions Prim-25 Not Detected posterior cardinal vein vascular sprouts
IHC flt4um200/um200 standard conditions Prim-25 Not Detected posterior cardinal vein vascular sprouts
Ab10-prox1 IHC y1Tg standard conditions Prim-25 corpuscles of Stannius
IHC Prim-25 lymphangiogenic sprout
IHC Prim-25 muscle pioneer
IHC Prim-25 posterior cardinal vein endothelial cell
IHC y1Tg ; flt4um200/um200 standard conditions Prim-25 corpuscles of Stannius
IHC Prim-25 lymphangiogenic sprout
IHC Prim-25 muscle pioneer
IHC Prim-25 posterior cardinal vein endothelial cell
Phenotype Details
Fish Conditions Stage Phenotype
flt4um131/um131 standard conditions Prim-25 posterior cardinal vein vascular sprouts Ab2-tek labeling absent, abnormal
Prim-25 posterior caudal vein vascular sprouts decreased amount, abnormal
Protruding-mouth intersegmental artery increased amount, abnormal
Protruding-mouth intersegmental vein decreased amount, abnormal
flt4um131/um131 ; y1Tg standard conditions Prim-25 lymphangiogenic sprout decreased amount, abnormal
Long-pec intersegmental vein EGFP expression absent, abnormal
Long-pec parachordal vessel absent, abnormal
Long-pec parachordal vessel vasculature development decreased occurrence, abnormal
flt4um159/+ standard conditions Prim-25 lymphangiogenic sprout decreased amount, abnormal
flt4um159/um159 standard conditions Prim-15 lymphangiogenic sprout decreased amount, abnormal
Prim-25 lymphangiogenic sprout decreased amount, abnormal
flt4um200/um200 standard conditions Prim-25 posterior cardinal vein vascular sprouts Ab2-tek labeling absent, abnormal
Prim-25 posterior caudal vein vascular sprouts decreased amount, abnormal
Protruding-mouth intersegmental artery increased amount, abnormal
Protruding-mouth intersegmental vein decreased amount, abnormal
vegfcum18/+ ; y1Tg standard conditions Prim-25 lymphangiogenic sprout decreased amount, abnormal
vegfcum18/um18 standard conditions Protruding-mouth intersegmental artery increased amount, abnormal
Protruding-mouth intersegmental vein decreased amount, abnormal
vegfcum18/um18 ; y1Tg standard conditions Prim-25 lymphangiogenic sprout decreased amount, abnormal
y1Tg ; flt4um200/+ standard conditions Prim-25 lymphangiogenic sprout decreased amount, abnormal
y1Tg ; flt4um200/um200 standard conditions Prim-25 lymphangiogenic sprout decreased amount, abnormal
Long-pec intersegmental vein EGFP expression absent, abnormal
Long-pec parachordal vessel absent, abnormal
Long-pec parachordal vessel vasculature development decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development