ZFIN ID: ZDB-FIG-161110-22
Cheng et al., 2016 - Vinculin b deficiency causes epicardial hyperplasia and coronary vessel disorganization in zebrafish. Development (Cambridge, England)   143(19):3522-3531 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Days 14-20
PHENOTYPE:
Fish:
Observed In:
Stage: Days 14-20

Fig. S8

Epicardial over-proliferation is prominent in v12 mutant. (A-B) Mef2 and PCNA immunostaining images show that increased proliferating cells in the v12 mutant myocardium. (C-D) Epicardial cells is labeled by Tg(tcf21:dsRed) transgenic reporter. Increased PCNA positive cells found in the epicardium region. (E-F) Increased proliferative cells in the endocardium of v12 mutant labeled by Tg(fli1a:EGFP) transgenic reporter. (G) v12mutant has more PCNA positive nuclei per 105μm2 than the wild type sibling. Data are presented as mean±s.e.m.two-tailed unpaired t-test, **, P<0.01. Scale bar: 20μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 pd37Tg standard conditions Days 14-20 epicardium IHC
vclbgd4Gt/gd4Gt; pd37Tg standard conditions Days 14-20 epicardium IHC
EGFP vclbgd4Gt/gd4Gt; y1Tg standard conditions Days 14-20 endocardium IHC
y1Tg standard conditions Days 14-20 endocardium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mef2 IHC WT standard conditions Days 14-20 myocardium
IHC vclbgd4Gt/gd4Gt standard conditions Days 14-20 myocardium
Ab2-pcna IHC pd37Tg standard conditions Days 14-20 epicardium
IHC vclbgd4Gt/gd4Gt; pd37Tg standard conditions Days 14-20 epicardium
IHC vclbgd4Gt/gd4Gt; y1Tg standard conditions Days 14-20 endocardium
IHC y1Tg standard conditions Days 14-20 endocardium
Ab5-pcna IHC WT standard conditions Days 14-20 myocardium
IHC vclbgd4Gt/gd4Gt standard conditions Days 14-20 myocardium
Phenotype Details
Fish Conditions Stage Phenotype
vclbgd4Gt/gd4Gt standard conditions Days 14-20 myocardium cell population proliferation increased occurrence, abnormal
vclbgd4Gt/gd4Gt; pd37Tg standard conditions Days 14-20 epicardium cell population proliferation increased occurrence, abnormal
vclbgd4Gt/gd4Gt; y1Tg standard conditions Days 14-20 endocardium cell population proliferation increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development