ZFIN ID: ZDB-FIG-161110-13
Cheng et al., 2016 - Vinculin b deficiency causes epicardial hyperplasia and coronary vessel disorganization in zebrafish. Development (Cambridge, England)   143(19):3522-3531 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Days 14-20 to Days 45-89
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 14-20 to Days 45-89

Fig. 6

pERK and pFAK are upregulated in the epicardium and endocardium of v12 mutant ventricles. (A-B') pErk1/2 expression (green) is increased in the endocardium of v12 mutants at 55 dpf compared with wild-type sibling. (C-D') pErk1/2 expression is increased in the epicardium and endocardium of v12 mutant at 15 dpf. MF20 staining (red) indicates cardiac muscles. (E-F') Tg(tcf21:dsRed) transgenic reporter indicates epicardium. Upregulated pErk1/2 colocalizes with RFP signal. (G) Co-immunoprecipitation shows that NVclb-EGFP is not associated with paxillin, which normally interacts with wild-type Vclb, when ectopically expressed in HEK293 cells. IP, immunoprecipitation; WB, western blot. Size markers (kDa) are shown to the left. (H-I') The localization of paxillin at the boundary between epicardium and myocardium is partially lost in the v12 mutant (L,L') compared with wild-type siblings (H,H'). (J-M) pFAK(Y576/577) expression is upregulated whereas total FAK expression remains unchanged in the epicardium and endocardium of the v12 mutant at 45 dpf (L,M) compared with wild-type sibling (J,K). Scale bars: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 pd37Tg standard conditions Days 14-20 epicardium IHC
vclbgd4Gt/gd4Gt; pd37Tg standard conditions Days 14-20 epicardium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT standard conditions Days 14-20 myocardium
IHC Days 45-89 myocardium
IHC vclbgd4Gt/gd4Gt standard conditions Days 14-20 myocardium
IHC Days 45-89 myocardium
Ab1-aldh1a2 IHC WT standard conditions Days 30-44 epicardium
IHC vclbgd4Gt/gd4Gt; pd37Tg standard conditions Days 30-44 epicardium
Ab2-ptk2 IHC WT standard conditions Days 45-89 endocardium endothelial cell
IHC Days 45-89 epicardium
IHC Days 45-89 heart vasculature
IHC vclbgd4Gt/gd4Gt standard conditions Days 45-89 endocardium endothelial cell
IHC Days 45-89 epicardium
IHC Days 45-89 heart vasculature
Ab9-mapk IHC WT standard conditions Days 14-20 endocardium
IHC Days 14-20 epicardium
IHC Days 45-89 endocardium
IHC pd37Tg standard conditions Days 14-20 heart
IHC vclbgd4Gt/gd4Gt standard conditions Days 14-20 endocardium
IHC Days 14-20 epicardium
IHC Days 45-89 endocardium
IHC vclbgd4Gt/gd4Gt; pd37Tg standard conditions Days 14-20 epicardium
Phenotype Details
Fish Conditions Stage Phenotype
vclbgd4Gt/gd4Gt standard conditions Days 14-20 endocardium ab9-mapk labeling increased amount, abnormal
Days 14-20 epicardium ab9-mapk labeling increased amount, abnormal
Days 45-89 endocardium ab9-mapk labeling increased amount, abnormal
Days 45-89 endocardium endothelial cell Ab2-ptk2 labeling increased amount, abnormal
Days 45-89 epicardium Ab2-ptk2 labeling increased amount, abnormal
Days 45-89 heart vasculature Ab2-ptk2 labeling increased amount, abnormal
vclbgd4Gt/gd4Gt; pd37Tg standard conditions Days 14-20 epicardium ab9-mapk labeling increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development