FIGURE

Fig. 3

ID

ZDB-FIG-161024-4

Publication

Nissim et al., 2016 - Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development

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Fig. 3

Nr5a2 is not required for the formation of pdx1 and ptf1a pancreas progenitors. (A) The area of pdx1 pancreas progenitors is similar across nr5a2^+/+, nr5a2^+/oz3, and nr5a2^oz3/oz3 embryos at 26 hpf. (B) No significant differences in pdx1 progenitor population size identified by a quantification of pdx1 expression area by genotype (p>3 per group; for nr5a2^+/+vs. nr5a2^+/oz3, p=0.6746; for nr5a2^+/+vs. nr5a2^oz3/oz3, p=0.6606; unpaired t-test). (C) pdx1 pancreas progenitors are formed at 48 hpf in nr5a2^+/oz3 and nr5a2^oz3/oz3 embryos; however, subtle difference in pdx1 progenitor budding is noted with variable penetrance across the nr5a2^oz3/oz3 population. (D) No significant differences in the size of the pdx1 pancreas progenitor pool were observed by quantification of expression area at 48 hpf (n>3 per group; for nr5a2^+/+vs. nr5a2^+/oz3, p=0.6024; for nr5a2^+/+vs. nr5a2^oz3/oz3, p=0.4178; unpaired t-test). (E) ptf1a progenitors are formed in nr5a2^+/oz3 and nr5a2^oz3/oz3 embryos by 48 hpf.

Expression Data

Genes:	pdx1 ptf1a
Fish:	WT nr5a2^oz3/oz3 nr5a2^oz3/+
Anatomical Term:	pancreatic bud
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.