ZFIN ID: ZDB-FIG-161024-2
Nissim et al., 2016 - Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development. Developmental Biology   418(1):108-23 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 1

nr5a2 is expressed in the developing endoderm and is required for exocrine pancreas formation. (A) Expression pattern of nr5a2 in the developing endoderm by in situ hybridization. nr5a2 appears in the developing liver bud (red arrow) by 30 hpf and the pancreas bud (blue arrow) by 36 hpf. After 84 hpf, it is expressed in the mature liver (red arrow) and exocrine pancreas (blue arrow). (B) Expression of nr5a2 in FACS isolated acinar cells from the Tg(trypsin:GFP) line at 72 hpf by qRT-PCR. (C) Expression of nr5a2 in FACS isolated hepatocytes from pooled Tg(fabp10a:GFP) embryos at 72 hpf by qRT-PCR (fold enrichment displayed as an average of technical triplicates). (D) Splice-site morpholino (MO) knockdown of Nr5a2 reduces the size of the exocrine pancreas by in situ hybridization for trypsin at 96 hpf. (E) nr5a2 MO injected embryos (96 hpf) have a reduced exocrine pancreas size (blue arrow) by histology and section in situ hybridization for trypsin (n=5). (F) FACS quantification of GFP+cells (% of control) from elastase:GFP reporter fish at 96 hpf demonstrates that MO knockdown of nr5a2 reduces the number of exocrine pancreas cells (n=20 per treatment; p<0.05; unpaired t-test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP zf578Tg standard conditions Protruding-mouth pancreatic acinar cell RTPCR
Protruding-mouth whole organism RTPCR
nr5a2 WT standard conditions Prim-15 liver ISH
Prim-25 liver ISH
Prim-25 pancreatic bud ISH
Long-pec exocrine pancreas ISH
Long-pec pancreatic bud ISH
Pec-fin liver ISH
Pec-fin pancreatic acinar cell ISH
Pec-fin pancreatic bud ISH
Protruding-mouth exocrine pancreas ISH
Protruding-mouth liver ISH
Day 4 exocrine pancreas ISH
Day 4 liver ISH
Day 5 exocrine pancreas ISH
Day 5 liver ISH
prss1 WT control Day 4 exocrine pancreas ISH
WT + MO2-nr5a2 standard conditions Day 4 exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-nr5a2 standard conditions Day 4 exocrine pancreas prss1 expression decreased distribution, abnormal
Day 4 exocrine pancreas decreased size, abnormal
gz2Tg + MO2-nr5a2 standard conditions Day 4 exocrine pancreas cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 418(1), Nissim, S., Weeksb, O., Talbot, J.C., Hedgepeth, J.W., Wucherpfennig, J., Schatzman-Bone, S., Swinburne, I., Cortes, M., Alexa, K., Megason, S., North, T.E., Amacher, S.L., Goessling, W., Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development, 108-23, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.