ZFIN ID: ZDB-FIG-160927-51
Zhao et al., 2016 - Enhanced angiogenesis, hypoxia and neutrophil recruitment during Myc-induced liver tumorigenesis in zebrafish. Scientific Reports   6:31952 Full text @ Sci. Rep.
Knockdown Reagent:
Anatomical Term:
Stage: Days 7-13
Knockdown Reagent:
Observed In:
Stage: Days 7-13

Fig. 5

Stimulation of tumorigenic liver growth by tumor-infiltrated neutrophils.

TO(Myc) and Tg(mpx:EGFP) double transgenic larvae were used for norpholino knockdown of Gcsfr to inhibit neutrophil differentiation. Morpholino oligonucleotides were injected into the embryos at 1-2-cell stage. (A-D) Images of the double transgenic larvae after injection of either MO(control) (A,C) or MO(gcsfr) (B,D). These injected embryos were either treated with Dox (C,D) or without Dox from 4 dpf to 7 dpf (A,B). The liver areas are circled. The original magnification was 20x. (E) Neutrophil counts in the liver. (F) Neutrophil density in the liver under different conditions. (G) Quantification of 2D liver size. The quantitative data were based on 5 samples per concentration group. Data are represented as mean ± SD. Asterisks indicate significant difference with P-value < 0.05 by unpaired t-test statistical analysis. Scale bars = 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP gz26Tg; rj30Tg + MO3-csf3r control Days 7-13 neutrophil IFL
gz26Tg; rj30Tg + MO3-csf3r chemical treatment by environment: doxycycline Days 7-13 neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz26Tg; rj30Tg control Days 7-13 liver increased size, abnormal
Days 7-13 liver neutrophil increased amount, abnormal
gz26Tg; rj30Tg + MO3-csf3r chemical treatment by environment: doxycycline Days 7-13 liver neutrophil amount, ameliorated
Days 7-13 liver size, ameliorated
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