ZFIN ID: ZDB-FIG-160927-50
Zhao et al., 2016 - Enhanced angiogenesis, hypoxia and neutrophil recruitment during Myc-induced liver tumorigenesis in zebrafish. Scientific Reports   6:31952 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Days 7-13

Fig. 4

Enhanced tumor infiltration of neutrophils by induced Myc expression and suppression of neutrophil infiltration by angiogenesis inhibitors.

TO(Myc) and Tg(mpx:EGFP) double transgenic larvae were generated with EGFP labeled neutrophils. (A-F) Images of the double transgenic larvae in the presence of the following chemicals: nil control (A), Dox (B), SU5416 (C), Dox+SU5416 (D), sunitinib (E) and Dox+sunitinib (F). The larvae were treated from 4 dpf to 7 dpf. In all images, the liver areas are circled by dashlines. The original magnification was 20x. (G) Neutrophil counts in the liver. (H) Neutrophil density in the liver. Neutrophil density was calculated as number of neutrophils per µm2. (I) Quantification of 2D liver size. The quantitative data were based on 10 samples per concentration group. Data are represented as mean ± SD. Astrisks indicate significant difference with P-value < 0.05 by unpaired t-test statistical analysis.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP rj30Tg ; gz26Tg chemical treatment by environment: doxycycline Days 7-13 neutrophil IFL
rj30Tg ; gz26Tg chemical treatment by environment: doxycycline, chemical treatment by environment: semaxanib Days 7-13 neutrophil IFL
rj30Tg ; gz26Tg chemical treatment by environment: doxycycline, chemical treatment by environment: sunitinib Days 7-13 neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rj30Tg ; gz26Tg chemical treatment by environment: doxycycline Days 7-13 liver increased size, abnormal
rj30Tg ; gz26Tg chemical treatment by environment: doxycycline Days 7-13 liver neutrophil increased amount, abnormal
rj30Tg ; gz26Tg chemical treatment by environment: doxycycline, chemical treatment by environment: semaxanib Days 7-13 liver neutrophil amount, ameliorated
rj30Tg ; gz26Tg chemical treatment by environment: doxycycline, chemical treatment by environment: sunitinib Days 7-13 liver neutrophil amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.