ZFIN ID: ZDB-FIG-160927-47
Zhao et al., 2016 - Enhanced angiogenesis, hypoxia and neutrophil recruitment during Myc-induced liver tumorigenesis in zebrafish. Scientific Reports   6:31952 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. 1

Enhanced angiogenesis in the liver by induction of transgenic Myc expression in TO(Myc) larvae.

Tg(Fli1:EGFP), LiPan and TO(Myc) zebrafish were crossed to generate triple transgenic zebrafish larvae. The liver was labeled by DsRed expression and blood vessel by EGFP expression. Triple transgenic larvae were treated with 30 µg/ml Dox from 3 dpf to 7 dpf and imaged by a confocal microscope. (A) A representative triple transgenic larva without Dox treatment. (B) A representative triple transgenic larva with Dox treatment. (C) Blood vessel density. Blood vessel density is defined by the ratio of blood vessel area (green) over the total liver area (as demarcated by DsRed expression) and measured by online ImageJ software. The quantitative data were based on 10 samples per group. The original magnification was 40x. Standard error bars are indicated and the two groups show significant difference with P-value < 0.05 by unpaired t-test statistical analysis. Scale bars = 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed gz26Tg ; gz15Tg ; y1Tg control Days 7-13 liver IFL
gz26Tg ; gz15Tg ; y1Tg chemical treatment by environment: doxycycline Days 7-13 liver IFL
EGFP gz26Tg ; gz15Tg ; y1Tg control Days 7-13 blood vessel IFL
gz26Tg ; gz15Tg ; y1Tg chemical treatment by environment: doxycycline Days 7-13 blood vessel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz26Tg ; gz15Tg ; y1Tg chemical treatment by environment: doxycycline Days 7-13 angiogenesis increased occurrence, abnormal
Days 7-13 liver increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.