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ZFIN ID: ZDB-FIG-160908-6
Yabe et al., 2016 - Mesp quadruple zebrafish mutant reveals different roles of mesp genes in somite segmentation between mouse and zebrafish. Development (Cambridge, England)   143(15):2842-52 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 6

Abnormal muscle development in the Mesp quadruple mutant. (A-C) Immunostaining of Engrailed (4D9) and Prox1 in the wild type (A) and Mesp quadruple mutant (B). Embryos were fixed at 24hpf. Graph showing the numbers of MPs, identified as the 4D9 and Prox1 double-positive cells, in each somite in the wild-type and Mesp quadruple mutant embryos (C). The number of MPs at 24hpf was counted in the 5-6 somites located in the most posterior part of the trunk region. A total of 16 somites were counted from 3 wild-type embryos and 23 somites from 4 mutant embryos. (D,E) Expression of smyhc in the wild-type and Mesp quadruple mutant embryos at 24hpf. After whole-mount in situ hybridization, the embryos were transversely sectioned at the middle level of the yolk tube. (F,G) Expression of cxcl12a in the wild-type and Mesp quadruple mutant embryos at 24hpf (100%, n=14). (H,I) Expression of pax7 in wild-type and Mesp quadruple mutant embryos in the most posterior region of yolk tube. Embryos were fixed at the 20-somite stage. Scale bar: 50┬Ám. (J-L) Cell-autonomous function of Mesp in superficial horizontal myoseptum formation. (J) Schema for the transplantation experiment. The wild-type cells were transplanted at the marginal zone of Mesp quadruple mutant embryos at the blastula stage. Although the expression of cxcl12a is rescued in the transplanted embryo at 24hpf (K,L), this expression is restricted to the donor cells (K′′,L′′). K and L show the rescued phenotype in different embryos. Although the rescued expression of cxcl12a was observed in a total of 42 cells in 10 embryos, this rescue occurred only in the donor cells in all cases.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcl12a WT standard conditions Prim-5 horizontal myoseptum ISH
mespbbkt1009/kt1009 ; mespabkt1002/kt1002 ; mespaakt1017/kt1017 ; mespbakt1004/kt1004 standard conditions Prim-5 horizontal myoseptum ISH
pax7a WT standard conditions 20-25 somites somite ISH
mespbbkt1009/kt1009 ; mespabkt1002/kt1002 ; mespaakt1017/kt1017 ; mespbakt1004/kt1004 standard conditions 20-25 somites somite ISH
smyhc1 WT standard conditions Prim-5 horizontal myoseptum ISH
Prim-5 slow muscle cell ISH
Prim-5 somite superficial region ISH
mespbbkt1009/kt1009 ; mespabkt1002/kt1002 ; mespaakt1017/kt1017 ; mespbakt1004/kt1004 standard conditions Prim-5 horizontal myoseptum ISH
Prim-5 slow muscle cell ISH
Prim-5 somite superficial region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-eng IHC WT standard conditions Prim-5 adaxial cell
IHC Prim-5 horizontal myoseptum
IHC mespbbkt1009/kt1009 ; mespabkt1002/kt1002 ; mespaakt1017/kt1017 ; mespbakt1004/kt1004 standard conditions Prim-5 adaxial cell
Ab5-prox1 IHC WT standard conditions Prim-5 adaxial cell
IHC mespbbkt1009/kt1009 ; mespabkt1002/kt1002 ; mespaakt1017/kt1017 ; mespbakt1004/kt1004 standard conditions Prim-5 adaxial cell
Phenotype Details
Fish Conditions Stage Phenotype
mespbbkt1009/kt1009 ; mespabkt1002/kt1002 ; mespaakt1017/kt1017 ; mespbakt1004/kt1004 standard conditions 20-25 somites somite pax7a expression decreased amount, abnormal
20-25 somites somite pax7a expression spatial pattern, abnormal
Prim-5 horizontal myoseptum absent, abnormal
Prim-5 horizontal myoseptum cxcl12a expression spatial pattern, abnormal
Prim-5 horizontal myoseptum smyhc1 expression spatial pattern, abnormal
Prim-5 muscle pioneer mislocalised medially, abnormal
Prim-5 slow muscle cell morphology, abnormal
Prim-5 somite shape, abnormal
Prim-5 somite superficial region smyhc1 expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development