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General Information
ZIRC
ZFIN ID: ZDB-FIG-160802-24
Xu et al., 2012 - Unique domain appended to vertebrate tRNA synthetase is essential for vascular development. Nature communications   3:681 Full text @ Nat. Commun.
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 6

Rescue experiments in zebrafish demonstrating the role of UNE-S in vascular development. (a) Illustration of the phenotype rescue experiment in zebrafish. The SerRS morpholino (SerRS-MO, ~5 ng per embryo) was injected into the yolk of zebrafish embryos (1- to 2-cell stage) to produce a model of intersegmental vessel (ISV) abnormality. Co-injection of SerRS-MO with the WT human SerRS mRNA (~250 pg per embryo) rescued the aberrant ISV branching. The ISV development is recorded at 72 h post fertilization and the abnormal ISV branches are indicated by red arrows. (b) Co-injection of SerRS-MO with the NLS-deleted Δ482-514 or NLSmut human SerRS mRNA did not rescue the aberrant ISV branching. (c) Co-injection of SerRS-MO with F383V human SerRS mRNA did not, whereas with F383V/D378R SerRS mRNA did, rescue the aberrant ISV branching. (d) The effect of SerRS nuclear localization on VEGFA transcription. Semiquantitative RT–PCR analysis to evaluate vefga mRNA levels in 72-hpf zebrafish embryos injected with SerRS-MO alone or SerRS-MO together with WT or mutant human SerRS mRNAs. Relative vegfa mRNA levels represented as fold increase (mean±s.e.m., n=3, *P<0.05 (Student′s t-test)) over uninjected control samples, after normalization to the levels of β-actin mRNA. Scale bars in this figure correspond to 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
vegfaa y1Tg standard conditions Protruding-mouth whole organism RTPCR
y1Tg + MO1-sars standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-sars standard conditions Protruding-mouth intersegmental vessel increased branchiness, abnormal
Protruding-mouth whole organism vegfaa expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.