ZFIN ID: ZDB-FIG-160802-24
Rescue experiments in zebrafish demonstrating the role of UNE-S in vascular development. (a) Illustration of the phenotype rescue experiment in zebrafish. The SerRS morpholino (SerRS-MO, ~5 ng per embryo) was injected into the yolk of zebrafish embryos (1- to 2-cell stage) to produce a model of intersegmental vessel (ISV) abnormality. Co-injection of SerRS-MO with the WT human SerRS mRNA (~250 pg per embryo) rescued the aberrant ISV branching. The ISV development is recorded at 72 h post fertilization and the abnormal ISV branches are indicated by red arrows. (b) Co-injection of SerRS-MO with the NLS-deleted Δ482-514 or NLSmut human SerRS mRNA did not rescue the aberrant ISV branching. (c) Co-injection of SerRS-MO with F383V human SerRS mRNA did not, whereas with F383V/D378R SerRS mRNA did, rescue the aberrant ISV branching. (d) The effect of SerRS nuclear localization on VEGFA transcription. Semiquantitative RT–PCR analysis to evaluate vefga mRNA levels in 72-hpf zebrafish embryos injected with SerRS-MO alone or SerRS-MO together with WT or mutant human SerRS mRNAs. Relative vegfa mRNA levels represented as fold increase (mean±s.e.m., n=3, *P<0.05 (Student′s t-test)) over uninjected control samples, after normalization to the levels of β-actin mRNA. Scale bars in this figure correspond to 100 µm.
Antibody Labeling Details
No data available
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Full text @ Nat. Commun.