ZFIN ID: ZDB-FIG-160725-5
Prabhudesai et al., 2016 - LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation. Journal of neuroscience research   94(8):717-35 Full text @ J. Neurosci. Res.
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 6

LRRK2 expression in wild-type embryos. A: LRRK2 is expressed throughout the brain, in the lens, in the heart, in the pronephros, and in the spinal cord (red). Neuronal labeling was visualized by GFP expression driven by the HuC promoter (green). Overlay of the red and green channels showing a mixture of overlap of signal (yellow) but also separate red and green signals demonstrating that LRRK2 is expressed both in Huc-GFP neurons and surrounding the neurons. Te, telencephalon; De, diencephalon; Mb, midbrain; Hb, hindbrain; Pn, pronephros; SC, spinal cord; L, lens; H, heart′ OB, olfactory bulb. B,C: Comparison of LRRK2 protein expression using immunofluorescence between wt (B) and LRRK2 translational MO-injected (C) embryos. LRRK2 protein expression is reduced in the LRRK2 morphants (C) compared with wt embryos (B). White arrows indicate examples of specific and punctate immunostaining in wt embryos. The background red staining in C appears higher than in B because the exposure was increased in C to verify less specific LRRK2 cross-reactivity. Scale bars = 100 µm in A-C; 50 µm in i,ii.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lrrk2 Ab2-lrrk2 AB standard conditions Long-pec diencephalon IHC
Ab2-lrrk2 Long-pec heart IHC
Ab2-lrrk2 Long-pec hindbrain IHC
Ab2-lrrk2 Long-pec lens IHC
Ab2-lrrk2 Long-pec midbrain IHC
Ab2-lrrk2 Long-pec olfactory bulb IHC
Ab2-lrrk2 Long-pec pronephric duct IHC
Ab2-lrrk2 Long-pec pronephros IHC
Ab2-lrrk2 Long-pec Rohon-Beard neuron IHC
Ab2-lrrk2 Long-pec spinal cord IHC
Ab2-lrrk2 Long-pec spinal cord neuron IHC
Ab2-lrrk2 AB + MO3-lrrk2 standard conditions Long-pec brain IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-lrrk2 standard conditions Long-pec brain lrrk2 expression decreased amount, abnormal
ZFIN wishes to thank the journal Journal of neuroscience research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Neurosci. Res.