ZFIN ID: ZDB-FIG-160725-1
Prabhudesai et al., 2016 - LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation. Journal of neuroscience research   94(8):717-35 Full text @ J. Neurosci. Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 1

LRRK2 knockdown by translational MO produces axis curvature defects, ocular abnormalities, and edema. A: Western blot analysis showing LRRK2 expression in wt embryos and in LRRK2 morphant embryos using a LRRK2-specific antibody normalized to GAPDH. B: Quantification of LRRK2 expression normalized to GAPDH. Y-axis shows LRRK2 expression in LRRK2 morphant embryos as a percentage of LRRK2 expression in wt embryos. n = 3 experiments for each group; 12 embryos in each group in each experiment. C: Lateral view of a 3-dpf LRRK2 morphant embryo with axis curvature defects (asterisk). Lateral view of a 3-dpf LRRK2 5-base mismatch MO-injected embryo (D) and a 3-dpf wt embryo both showing a wt phenotype and straight tail (E). F: Dorsal view of a 3-dpf LRRK2 morphant embryo with axis curvature defects (asterisk) and with edema in otic vesicles (arrow in main image) and lens (arrow in inset). Dorsal view of a 3-dpf LRRK2 5-base mismatch MO-injected embryo (G) and a 3-dpf wt embryo both with normal lenses, otic vesicles, and a straight tail (H). I: Lateral view of a 6-dpf LRRK2 morphant embryo showing edema around the eyes and ventral curvature defects (bent tail; pound sign). Lateral view of a 6-dpf LRRK2 5-base mismatch MO-injected embryo (J) and a 6-dpf wt embryo both showing normal eye development and a straight tail (K). L: Dorsal view of a 6-dpf LRRK2 morphant showing distinct edema around the eyes (arrows). Dorsal view of a LRRK2 5-base mismatch MO-injected embryo (M) and a 6-dpf wt embryo both showing normal eyes (N). O: Ventral view of 3-dpf LRRK2 morphant showing coloboma in one of the eyes (arrow). P: Ventral view of a LRRK2 5-base mismatch MO-injected embryo showing normal eyes. n = 2,932 for LRRK2 translational morphants, n = 2,100 for LRRK2 5-base mismatch controls MO, n = 2,142 for wt. ***P < 0.001 (Student′s t-test). Scale bars = 0.5 mm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lrrk2 Ab2-lrrk2 AB standard conditions Protruding-mouth whole organism WB
Ab2-lrrk2 AB + MO3-lrrk2 standard conditions Protruding-mouth whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-lrrk2 standard conditions Protruding-mouth eye decreased size, abnormal
Protruding-mouth eye malformed, abnormal
Protruding-mouth lens edematous, abnormal
Protruding-mouth optic fissure open, abnormal
Protruding-mouth otic vesicle edematous, abnormal
Protruding-mouth post-vent region bent, abnormal
Protruding-mouth whole organism lrrk2 expression decreased amount, abnormal
Day 6 eye decreased size, abnormal
Day 6 post-vent region bent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of neuroscience research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Neurosci. Res.