ZFIN ID: ZDB-FIG-160706-37
Kwon et al., 2016 - In vivo modulation of endothelial polarization by Apelin receptor signalling. Nature communications   7:11805 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Pec-fin
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. S7

apln mutants show reduced endothelial cell numbers.

(a-b) in situ hybridization of 48 hpf wild-type (a) and apln -/- (b) embryos for apela expression. The numbers of embryos examined are indicated in the top right corner of the images. (c, d) Quantitative analysis of EC numbers per three somites in 72 hpf aplnrb+/- (c) and apln -/- (d) larvae. The numbers of larvae (n) and ECs (n′) are indicated above the graph. (e-f′) Confocal images (lateral views) of 60 hpf Tg(fli1a:EGFP); Tg(-0.8flt1:RFP) wild-type (e, e′) and aplnrb+/- (f, f′) embryos. (g-i) in situ hybridization of 48 hpf wild-type (g), aplnrb +/- (h) and aplnrb -/- (i) embryos for vegfr3 expression. (j-k′) Confocal images (lateral views) of 60 hpf Tg(fli1a:EGFP); Tg(EPV.TP1-Mmu.Hbb:hist2h2l97 mCherry) wild-type (j, j′) and aplnrb+/- (k, k′) embryos. The numbers of embryos examined are in the top right corner of the images (e′, f′, g-i, j′, k′). Anterior to t 98 he left, dorsal to the top. Scale bars, 60 µm (a-b, g-i) , 20 µm (e-f′, j-k′). DA, dorsal aorta; PCV, posterior cardinal vein. Error bars, SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apela AB standard conditions Long-pec whole organism ISH
aplnmu267/mu267 standard conditions Long-pec whole organism ISH
EGFP aplnrbmu270/+ ; s939Tg ; y1Tg standard conditions Pec-fin vasculature IFL
hu5333Tg; y1Tg standard conditions Pec-fin vasculature IFL
y1Tg ; hu5333Tg ; aplnrbmu270/+ standard conditions Pec-fin vasculature IFL
y1Tg ; s939Tg standard conditions Pec-fin vasculature IFL
flt4 AB standard conditions Long-pec posterior cardinal vein ISH
aplnrbmu270/+ standard conditions Long-pec posterior cardinal vein ISH
aplnrbmu270/mu270 standard conditions Long-pec posterior cardinal vein ISH
mCherry aplnrbmu270/+ ; s939Tg ; y1Tg standard conditions Pec-fin whole organism IFL
y1Tg ; s939Tg standard conditions Pec-fin whole organism IFL
RFP hu5333Tg; y1Tg standard conditions Pec-fin vasculature IFL
y1Tg ; hu5333Tg ; aplnrbmu270/+ standard conditions Pec-fin vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aplnrbmu270/+ standard conditions Protruding-mouth endothelial cell decreased amount, abnormal
aplnrbmu270/mu270 standard conditions Protruding-mouth endothelial cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.