ZFIN ID: ZDB-FIG-160511-12
DeRossi et al., 2016 - trappc11 is required for protein glycosylation in zebrafish and humans. Molecular biology of the cell   27(8):1220-34 Full text @ Mol. Biol. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 6

Atorvastatin is synthetically lethal with trappc11 mutation. (A) Transgenic WT and trappc11-mutant embryos expressing a liver-specific marker (Tg(fabp10:dsRed)) were treated with DMSO or the HMG-CoA reductase inhibitor, Atv, between 3 and 5 dpf at the concentrations indicated and analyzed for gross morphology and lethality. (B) Livers were collected from WT (n = 7 clutches), trappc11 (n = 8 clutches), and 5 µM Atv–treated WT embryos between 3 and 5 dpf (n = 7 clutches) and subjected to qPCR to analyze UPR gene expression used to calculate PC1. p values were calculated with Student’s t test. n.s., not significant. (C) Livers from larvae treated with DMSO or 1 µM Atv from 3 to 5 dpf (n = 8 clutches) were collected and analyzed at 5 dpf for the expression of genes involved in N-linked glycosylation. Atorvastatin-induced fold increases are shown relative to DMSO-treated samples.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alg2 AB/TU chemical treatment: atorvastatin Day 5 liver RTPCR
alg5 AB/TU chemical treatment: atorvastatin Day 5 liver RTPCR
alg9 AB/TU chemical treatment: atorvastatin Day 5 liver RTPCR
dpagt1 AB/TU chemical treatment: atorvastatin Day 5 liver RTPCR
gmppb AB/TU chemical treatment: atorvastatin Day 5 liver RTPCR
hmgcs1 AB/TU chemical treatment: atorvastatin Day 5 liver RTPCR
pmm2 AB/TU chemical treatment: atorvastatin Day 5 liver RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU chemical treatment: atorvastatin Day 5 liver alg2 expression increased amount, abnormal
Day 5 liver alg5 expression increased amount, abnormal
Day 5 liver alg9 expression increased amount, abnormal
Day 5 liver dpagt1 expression increased amount, abnormal
Day 5 liver gmppb expression increased amount, abnormal
Day 5 liver hmgcs1 expression increased amount, abnormal
Day 5 liver pmm2 expression increased amount, abnormal
gz15Tg ; trappc11hi1532bTg/hi1532bTg chemical treatment: atorvastatin Day 5 liver decreased size, abnormal
Day 5 response to endoplasmic reticulum stress increased occurrence, abnormal
Day 5 whole organism dead, abnormal
Day 5 whole organism decreased life span, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular biology of the cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol. Biol. Cell