header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-160329-22
Griffin et al., 2016 - Ferredoxin 1b (Fdx1b) is the essential mitochondrial redox partner for cortisol biosynthesis in zebrafish. Endocrinology   157(3):1122-34 Full text @ Endocrinology
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 4

Zebrafish fdx1bUOB205 homozygous mutants are glucocorticoid deficient.

A, fdx1bUOB205 homozygous mutants have impaired visual-mediated adaption. When exposed to a lighter environment, fdx1bUOB205 homozygous mutants are darker in appearance when compared with control siblings, which had a least 1 wild-type allele (fdx1b+/+ or fdx1bUOB205/+). B, Cortisol concentrations were determined from fdx1bUOB205 homozygous mutant larvae (fdx1bUOB205) and sibling controls (sib) under basal and stressed conditions. Cortisol was not detected (nd) from fdx1bUOB205 homozygous mutants under basal conditions. Concentrations were determined from 3 independent replicates and are expressed as picograms per larva (mean ± SD). C, Treatment of fdx1bUOB205 homozygous mutants with the glucocorticoid agonist dexamethasone (DEX) restored the light adaptation phenotype in contrast to ethanol-treated controls (EtOH). mRNA expression of glucocorticoid response genes (D) pck1 and (E) fkbp5 in fdx1bUOB205 homozygous mutants and control siblings under basal and stressed conditions. Graphs represent mean relative expression ± SEM (normalized to gapdh). F, Quantitative real-time expression of pomca in fdx1bUOB205/UOB205 mutants relative to control siblings. Statistical analysis was performed using one-way ANOVA; *, P < .05; **, P < .01; ***, P < .001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fkbp5 WT standard conditions Day 4 whole organism RTPCR
WT isotonic Day 4 whole organism RTPCR
fdx1buob205/uob205 standard conditions Day 4 whole organism RTPCR
fdx1buob205/uob205 isotonic Day 4 whole organism RTPCR
pck1 WT standard conditions Day 4 whole organism RTPCR
WT isotonic Day 4 whole organism RTPCR
fdx1buob205/uob205 standard conditions Day 4 whole organism RTPCR
fdx1buob205/uob205 isotonic Day 4 whole organism RTPCR
pomca WT standard conditions Day 4 whole organism RTPCR
fdx1buob205/uob205 isotonic Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT isotonic Day 4 whole organism fkbp5 expression increased amount, abnormal
Day 4 whole organism pck1 expression increased amount, abnormal
fdx1buob205/uob205 standard conditions Day 4 whole organism pck1 expression decreased amount, abnormal
Day 4 whole organism fkbp5 expression decreased amount, abnormal
Day 5 cortisol biosynthetic process disrupted, abnormal
fdx1buob205/uob205 isotonic Day 4 whole organism pck1 expression decreased amount, abnormal
Day 4 whole organism fkbp5 expression decreased amount, abnormal
Day 4 whole organism pomca expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Endocrinology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Endocrinology